章基因组学与比较基因组学.pptVIP

祝同学们新年快乐！考试顺利！ * * * * * * * * * * Positional cloning is powerful, but it has also been extremely tedious. When the approach was first proposed in the early 1980s9, a researcher wishing to perform positional cloning had to generate genetic markers to trace inheritance; perform chromosomal walking to obtain genomic DNA covering the region; and analyse a region of around 1 Mb by either direct sequencing or indirect gene identification methods. The first two barriers were eliminated with the development in the mid-1990s of comprehensive genetic and physical maps of the human chromosomes, under th