RNA干扰载体构建.pdfVIP

Chapter 40 Construction of Hairpin RNA-Expressing Vectors for RNA-Mediated Gene Silencing in Fungi Shaobin Zhong, Yueqiang Leng, and Melvin D. Bolton Abstract RNA-mediated gene silencing is one of the major tools for functional genomics in fungi and can be achieved by transformation with constructs that express hairpin (hp) RNA with sequences homologous to the target gene(s). To make an hpRNA expression construct, a portion of the target gene can be amplifi ed by PCR and clone