用于检测老年痴呆症磁共振对比剂的研究.pdf

Preparation of magnetic nanoparticles as potential probe to image Alzheimer’s Amyloid plaque Liu Zuli, Du Guihuan, Yao Kailun, Xia Xing Department of Physics, Huazhong University of Science and Technology(HUST), Wuhan, China (430074) Abstract The contrast agent contained magnetic nanoparticles and amyloid-β (Aβ) peptide was synthesized by co-deposition and surface modification. The characterizations of the contrast agent were studied by vibrating sample magnetometer (VSM) and transmission electron microscopy (TEM). The in vivo imaging was investigated by imaging the brain of AD transgenic mice under magnetic resonance imaging device (MRI). The results showed the contrast agent is about 5nm in size and has good senile plaques enhancement by MRI. Keywords ：magnetic nanoparticles, contrast agent, Alzheimer’s disease ，Amyloid plaque 1 Introduction The clinical diagnosis of Alzheimer’s disease (AD) mostly depends on the evaluation of the patient, which is influenced largely by the doctor’s experience. The only definitive way to diagnose AD at present is demonstrating insoluble aggregation of amyloid-β peptide called senile plaque in postmortern, because there is a consensus among many investigators that celebral accumulation of the amyloid-β(Aβ) protein represents an early and necessary step in pathogenesis and is therefore worthy of therapeutic attack. Antibodies, congo red derivatives and other amyloid-specific dyes have been used to make detective agents to image AD[1 －5]. The work of Wen