难降解有机物对白腐真菌P450的诱导及P450的作用.pdfVIP

中国环境科学 2009,29(4) ：407~412 China Environmental Science 难降解有机物对白腐真菌P450 的诱导及P450 的作用 1 1* 1,2 1 宁大亮 ,王 慧 ,王立华 ,丁 昶 (1.清华大学环境科学与工程系,环境模拟与污染控制国家重点联合实验 室,北京 100084 ；2.河北北方学院理学院,河北 张家口 075000) 摘要：实验考察了多环芳烃和氯酚类化合物对黄孢原毛平革菌 P450 的诱导作用及其P450 对生物降解的影响.结果表明,萘、菲、2,4-二氯 酚、五氯酚能够诱导该真菌 P450,使微粒体 P450 比浓度达到 37~137pmol/mg.在营养限制条件下,菲诱导的 P450 是营养丰富条件的 5.7 倍;P450 抑制剂的作用使萘、菲、芘、2,4-二氯酚的降解效率下降 36%~100%,但是对 2,4,6-三氯酚和五氯酚的降解未产生影响.在营养丰 富条件下,P450 抑制剂的作用使萘、菲、芘、2,4,6-三氯酚的降解效率下降 47%~100%,使五氯酚的降解效率增大 57%,但对 2,4-二氯酚的 降解未产生影响. 关键词：白腐真菌；P450 ；多环芳烃；氯酚；诱导 中图分类号：X172 文献标识码：A 文章编号：1000-6923(2009)04-0407-06 Induction and function of cytochrome P450 in degradation of refractory organic chemicals by Phanerochaete 1 1* 1,2 1 chrysosporium. NING Da-liang , WANG Hui , WANG Li-hua , DING Chang (1.State Key Joint Laboratory of Environment Simulation and Pollution Control, Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China ；2. College of Science, Hebei North University, Zhangjiakou 075000, China). China Environmental Science, 2009,29(4)：407~412 Abstract ：The involvement of P450 in degradation of polycyclic aromatic hydrocarbons and chlorophenols by white rot fungus Phanerochaete chrysosporium was investigated. The significant induction of P450s by naphthalene, phenanthrene, 2,4-dichlorophenol and pentachlorophenol were observed. The specific concentrations of microsomal P450s were up to 37~137 pmol/mg in the induced cells. The content of phenanthrene-induced P450 from nutrient-limited culture was 5.7 times higher than that from nutrient-sufficient culture. Piperonyl butoxide (PB), a P450 inhibitor, markedly inhibited the degradation of naphthalene, phenanthrene and pyrene in both nutrient-limited and nut