《The Variety Selection and Testing for Effect on HepG-2 Cell Line of Dioscorea membranacea Pierre （Hua Khao Yen Tai） Extract Using Molecular Genetics.》.pdf

April2014，Vo1．8，No．4，PP．322·328 JournalofLifeSciences，ISSN 1934-7391，USA 钏 薯H TheVarietySelectionandTestingforEffectonHepG-·2 CellLineofDioscoreamembranaceaPierre(HuaKhao YenTai)ExtractUsingMolecularGenetics NantanaJaengsuwan andArunpornItharat ，．DepartmentofAppliedBiology,ThepsatriRajabhatUniversity,Mueang,LopBuri15000，Thailand 2．DepartmentofAppliedThaiTraditionalMedicine，FaculytofMedicine，ThammasatUniversity，Klongluang,Patumtani12120 Thnilnnd Received：December03，2013／Accepted：January23，2014／Published：April30，2014 Abstract：Inthisresearch，DioscoreamembranaceaPierrewasstudiedbyusing30samplesfromKhaoRuakSub．district，ChaiBadan District，LopBuriProvince，inThailand．Inthisresearch，somemorphologywasstudiedincludingshapes，leaves，stem colors， epidermalcells，stomatasizesandstipules．Tostudythegeneticrelationships，theAFLPtechniqueandcomputerprogram wereused． TheDioscoreamembranaceaPierrewasclassifiedinto2groupsaccordingtoitsphylogenetictype：thefirstgroupwas“HuaKhaoYen Tai—Nuca”(SmilaxcorbulariaKunth)，andthesecondgroupincluded30furthersamplesofHuaKhaoYenTaifDioscorea membranaceaPierre)．Theethanoliccrudeextractwasalsoappliedtotesttheanti-proliferativeactivityintheliverhepatocellular carcinoma(HepG2)celllineswhichillustratesthecharacteristicsofapoptosis：eellshrinkage．membraneblebbingandnuclear condensation．TheexpressionofBaxgeneisincreasedmorethanthatofthecontrolgroupwhileBcl一2genewhichisanti．apoptoticis decreased．Furthermore．theresultofwestern blotanalysisrevealstheup．regulationofBaxproteinanddown．regulationofBcl一2 proteinwhencomparedwithuntreatedcells．ThismightindicatethatethanoliccrudeextractsofHuaKhaoYenTaicouldinduce apoptosisandanti—proliferativeonHepG2eelIlines．Theresultsalsorevealedthatsomemorphologycannotbeusedtopredictwhich DioscoreamembranaceaPierreplantswouldbemosteffective． Keywords：DioscoreamembranaceaPierre，morphology，AFLPtechnique，apop