细胞生物学课件（费晓方）06Chapter5 II2010.pptVIP

Molecular basis of phosphorylation: ATP synthase Direct experimental evidence supporting the rotational catalysis. The ATP synthase is a reversible coupling device Other roles for the proton-motive force in addition to ATP synthase C. Mithchell’s Chemiosmotic theory (1961) * The structure of the ATP synthase F1 particle is the catalytic subunit; The F0 particle attaches to F1 and is embedded in the inner membrane. F1: 5 subunits in the ratio 3?:3?:1?:1?:1? F0: 1a：2b：12c  Figure?14-6?Fractionation of purified mitochondria into separate components.????These techniques have made it p