08肠道感染病毒.pptVIP

Chapter 21 ? source of infection (patients and carrier) ? cause poliomyelitis by fecal-oral route ? clinical findings of poliomyelitis 4.Laboratory diagnosis  of enteroviruses Isolation and identification of virus Detection of viral antibody and RNA 4. Diagnosis of infection * * Human enterovirus Viruses of causing acute gastroenteritis Poliovirus Coxsackievirus ? group A : types 1~22, 24 ? group B : types 1~6 Enteric cytopathogenic human orphan(ECHO) virus New enteroviruses (types 1~3) (31 types) (types 68~71) of enterovirus ? Shape and structure ?d=24~30 nm, spherical and naked virus with icosahedral nucleocapsid polyprotein ↓ VP1~VP4 and functional protein 衣壳蛋白(VP1与受体结合) ?genome:+ssRNA 1.Biological properties ? Cultivation ? Rresistance (stronger) (pH3~9稳定,污水和粪便中可存活数月) ? Type-specific antigen ? D(density)/N(native) antigen ? C (coreless) antigen ? primary cell cultureCPE ? animal inoculation (A1,A19,A22) 2.Pathogenicity of enteroviruses ? Replicate in the intestinal tract and spread through bloodstream to target organs ? Via fecal-oral route cause a variety of diseases or syndromes (夏秋季为主要流行季节) 不同肠道病毒可引起相同的临床症状,同一种病毒可引起几种不同的临床疾病 P275~276表 (通常有二次病毒血症) from subclinical infection to severe infection of CNS ⑴ Poliovirus (neurotropic virus) *subclinical infection(90%) *abortive infection (5%) *nonparalytic poliomyelitis/aseptic meningitis(1-2%) *paralytic poliomyelitis(0.1%-2%) nonparalytic : paralytic=100~1000 : 1 pathogen of poliomyelitis(脊髓灰质炎或小儿麻痹症) ⑵Coxsackievirus and ECHO virus ⑶ Enterovirus 71 cause a variety of diseases or syndromes (such as aseptic me