Bnm1, a Brassica pollen-specific gene：（Bnm1,芸苔属植物pollen-specific基因）.pdfVIP

Plant Molecular Biology 34: 603–611, 1997. 603 c 1997 Kluwer Academic Publishers. Printed in Belgium. Bnm1, a Brassica pollen-specific gene 1 2 3 4 2 Brian K. Treacy , Jiro Hattori , Isabelle Prud’homme , Eric Barbour , Kim Boutilier , Chris L. Baszczynski4 3 1 2; , Bin Huang , Douglas A. Johnson and Brian L. Miki 1Ottawa-Carleton Institute of Biology, University of Ottawa, Ottawa, Ontario, Canada K1N 6N5; 2Eastern Cereal  and Oilseed Research Center, Agriculture and Agri-Food Canada, Ottawa, Ontario, Canada K1A OC6 ( author for correspondence); 3Pioneer Hi-Bred Production Ltd, Mississauga, Ontario, Canada L7G 4S7; 4Pioneer Hi-Bred International Inc., Trait and Technology Division, Johnston, IO 50131-1004, USA Received 6 November 1996; accepted in revised form 6 March 1997 Key words: Brassica, GUS, pollen promoter, tissue culture, transgenic Abstract cDNA and genomic clones of a new pollen-specific gene, Bnm 1, have been isolated fromBrassica napus cv. Topas. The gene contains an open reading frame of 546 bp and a single intron of 362 bp. A comparison of the deduced amino acid sequence with sequences in data banks did not show similarity with known proteins. Northern blot analysis of developing pollen showed that Bnm 1 mRNA was first detected in bicellular pollen and accumulated to higher levels in tricellular pollen. Bnm 1 mRNA was not detected in leaves, stems, roots, pistils, seeds or pollen-derived embryos. RNA in situ hybridization of whole flower buds confirmed that Bnm 1 was pollen-spe