Bone Morphogenetic Protein-2 Stimulates Differentiation of Cultured Spinal Ligament Cells from Patients with Ossification of the Posterior Longitudinal Ligament：（骨形成Protein-2刺激分化培养脊柱韧带骨化患者的细胞后纵韧带）.pdfVIP

Calcif Tissue Int (1997) 60:291–296 © 1997 Springer-Verlag New York Inc. Bone Morphogenetic Protein-2 Stimulates Differentiation of Cultured Spinal Ligament Cells from Patients with Ossification of the Posterior Longitudinal Ligament T. Kon,1 M. Yamazaki,1 M. Tagawa,2 S. Goto,1 A. Terakado,1 H. Moriya,1 S. Fujimura2 1Department of Orthopaedic Surgery, School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260, Japan 2Department of Biochemistry, School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260, Japan Received: 1 April 1996 / Accepted: 19 July 1996 Abstract. Ossification of the posterior longitudinal liga- idiopathic skeletal hyperostosis (DISH) [2]; the former is ment (OPLL) of the spine is characterized by heterotopic characterized by the ossification of the anterior longitudinal bone formation occurring in spinal ligament, causing severe ligament and the latter represents a disease of systemic liga- compression myelopathy. In order to investigate the mecha- mentous ossification including the spinal ligaments. nism of OPLL development, we isolated spinal ligament Since a number of cases with neurological disorders due cells from OPLL patients as well as non-OPLL patients, and to OPLL have been reported in Japan, a special study group established 10 OPLL cell lines and 7 non-OPLL cell lines, on OPLL has been organized under government sponsor- respectively. We analyzed the effects of bone morphoge- ship [3]. Recent advances in cell culture techniques enable netic protein-2 (BMP-2) on these cells with respect to al- us to isolate cells from spinal ligaments and maintain them kaline phosphatase (AP) activity, DNA syn