pYTAC747HsacB载体的培养与功能分析.docVIP

pYTAC747H/sacB载体的培养与功能分析 *，肖炎凤，吴东旺，叶玉平，谭啸，彭迪 （湖南工程学院化学化工学院, 湖南 湘潭 411104） 摘要：通过对pYLTAC747H/sacB载体进行优化培养，绘制其生长曲线，用碱裂法小量提取TAC载体DNA，琼脂糖凝胶电泳分离载体DNA，研究了菌体活性及pYLTAC747H/sacB载体的一系列性能，获得了pYLTAC747H/sacB载体的一系列数据，可以作为载体pYLTAC747H/sacB培养条件的优化，标记筛选等的依据。pYTAC747H/sacB，大肠杆菌DH5α，卡那霉素ulture and Functional Analysis of pYTAC747H/sacB Vector Cao Hui Xu Yueyu* Xiao Yanfeng Wu Dongwang Ye Yuping Tan Xiao Peng Di (Chemistry and Engineering Department of Hunan Engineering Institute, Xiangtan 411104, China) Abstract: By culture and functional analysis of pYTAC747H/sacB vector，we can received a series of data of v