彗星实验操作步骤(DNA损伤检测).pdf

Chapter 6 The Comet Assay: A Sensitive Genotoxicity Test for the Detection of DNA Damage and Repair Günter Speit and Andreas Rothfuss Abstract The comet assay (single-cell gel electrophoresis) is a simple and sensitive method for studying DNA damage and repair. In this microgel electrophoresis technique, a small number of cells suspended in a thin agarose gel on a microscope slide is lysed, electrophoresed, and stained with a fluorescent DNA-binding dye. Cells with increased DNA damage display increased