02 培养与显微.pptVIP

Chapter 2 Pure Culture Technology Microscopy  2.1 Isolate Pure Culture Technology Small in size is the basic character of microbes Colony form in studing and conserving Pure culture is the first step in microbiological study Pure culture result in repeatable outcomes 2.1.1 Aseptic technique Autoclave 2.1.2 Isolate and pure culture bysolid medium 单个微生物在适宜的固体培养基表面或内部生长、繁殖到一定程度可以形成肉眼可见的、有一定形态结构的子细胞生长群体，称为菌落（colony） 当固体培养基表面众多菌落连成一片时，便成为菌苔（lawn） Pour plate method (稀释倒平板法) Spread plate method (涂布平板法) Streak plate method (平板划线法) Anaerobic culture method (厌氧培养法) Anaerobic Culture Metho