工作场所空气中臭氧的分光光度测定法.doc

工作场所空气中臭氧的分光光度测定法 张学忠荣县预,河北玉田 064100; 唐山市疾病预防控制中心,河北唐山 063000;) [摘要] 目的:建立作业场所空气中臭氧的测定新方法. 方法: 空气中臭氧与丁子香酚(4-烯丙基-2-甲氧基苯酚)生成的甲醛,在吸收液在与4-氨基-3-联-5-巯-1,2,4-三氮杂AHMT）缩高碘酸钾红6-巯-5-三氮杂[4,3-b]-S-四氮杂550nm测0.013-0. 492mg/m3内,相关系数r=0.9997,最低检出浓度为0.20μg/5mL，加标回收率在92.3%-97.7%之间。结论：该方法灵敏快速,不用有毒和致癌物质，结果准确可靠，重现性好，可作为作业场所中臭氧的测定方法。 [关键词]臭氧；作业场4-氨基-3-联-5-巯-1,2,4-三氮杂 Determining ozone in air of workshop by spectrophotometry ZHANG Xue-zhong1,XING Da-rong2, XIA Xiao-Li1 (1.Yutian Center for disease Control and Prevention,Yutian 064100,China;2. Tangshan Center for Disease Control and Prevention, Tangshan 064100,China) [Abstract] Objective: To establish a determination method of ozone in air of workshop by spectrophotometry. Methods: Formaldehyde Which was made by ozone in air reacting with eugenol(4-sllyl-2-methoxy phenlo) reacted with 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole(AHMT) to purplish red compound 6-mercapto-S-triazole[4,3-b] –S-tetrazole , which could be measured at wavelength 550nm by spectrophotometry. Results: The linearity ranged between 0.013-0.492mg/m3 .The recovery rate 92.3%-97.7% The lowest determination limit were 0.20μg/5mL . Conclusion: The method is simple,easy and highly toxic substance not used in determining course.And determining result is exact,reliable and good. [Key words]oaone；eugenol；spectrophotometry；4-amino-3-hydrazino-5-mercapto-1,2,4-triazole 臭氧是一种淡蓝色刺激性有毒气体对呼吸道及肺有强烈刺激作用引起支气管炎和肺水肿毒神经，记忆力退；诱发淋巴细胞染色体病变，儿畸形。空气中的氧被破坏生成臭氧。存241.1 仪器与试剂 T6型分光光度计氢钾钠AHMT溶液:0.25g AHMT溶于0.5mol/L盐高碘酸钾1.5g高碘酸钾0.2mol/L氢钠标100mg/L(国环护总标样,104107), 临用时释1.00μg/mL的标[作者简介] 张学忠(1968-)学师从卫检验1.2 测定方法 1.2.1 标准曲线绘制 分别吸取1.00μg/mL甲醛标ml于10ml比色管中，补吸收液至5.0ml,加1.0mL AHMT溶液，混匀,放置20min, 加0.5mL高碘酸钾,混匀,放置5min。用20mm比色皿,以试剂参550nm处测Y=0.2754X-0.001(r= 0.9997) 1.2.2 样品采集品 现场采样按GBZ159-2004执行。两只大型气泡吸收管串联，前管加1.0mL丁子香酚,后管加10.0mL吸收液,以2L/min流量采集15min空气样品。对照试验:将加好丁子香酚及吸收液的大型气泡吸收管串联带至现场,除不连接采样器采集空气外,其它操作同样品,作为样品空白对照。 采样后，立即封闭吸收管进出气口，置清洁容器内运输和保存。 1.2.3 样品处理 用采过样的吸收液洗涤吸收管进气管3次，摇匀后吸取5.0ml于10ml比色管中，供测定。若