60%VO2max运动对血液TLR-4及其诱导产物的影响.doc

  60%VO2max 运动对血液 TLR-4 及其诱导 产物的影响# 5 10 15 20 25 30 35 40 45  摘要：目的：研究 60%VO2max 运动对血液 TLR-4 mRNA 表达和蛋白水平及其诱导产物的 影响，通过对 60%VO2max 运动对免疫功能调节的观察，探讨运动与免疫的关系，对大众健 身等方面的研究提供理论依据。 方法：对 10 名非体育专业大学生进行 60%VO2max 的跑步机运动，在运动前，运动 后 0、1、2、24 小时抽取静脉血，测定血液中白细胞表面 TLR-4 mRNA 表达和血清 TLR-4 蛋白水平，血清中 NF-к B，促炎因子 IL-6，TNF-α 和抗炎因子 IL-4 的蛋白水平。 结果：(1) 60%VO2max 运动后即刻的血液中白细胞表面 TLR-4 mRNA 表达和血清 TLR-4 蛋白水平下降，血清 NF-к B、IL-4、TNF-α 蛋白水平上升，血清 IL-6 蛋白水平有先 升后降的趋势；除了 TLR-4 mRNA 表达，其他指标都在 24 小时后恢复运动前水平。 结论：(1) 60%VO2max 运动能引起白细胞表面 TLR-4 mRNA 表达和血清 TLR-4 蛋白 水平的下降，血清 NF-к B、TNF-α 、IL-4 蛋白水平的上升。(2)60%VO2max 运动对有关各 免疫指标的影响，在运动后 24 小时基本恢复到运动前状态。 关键词：运动人体科学；Toll 样受体 4；免疫；运动 中图分类号：Q599 Effects of 60%VO2max exercise on the expression of blood TLR-4 and its induced products QIU Tian1, WANG Xintang2, ZHANG Aifang3 (1. Sports School, Longyan University, Longyan, Fujian ; 364012 2. The Science Research Center,Beijing Sport University，Beijing 100084; 3. Human movement science School,Beijing Sport University,Beijing 100084) Abstract: Objective: Study the effects of 60%VO2max exercise on Toll receptor 4 mRNA expression,the serum protein level and its induced products influence, discuss the relationship between exercise and immunity,provide a theoretical help to public health and some other sport fileds. Methods: 10 non-sports major's college students were in 60%VO2max exercise,take blood before exercise and after exercise 0,1,2,24 hours from venous blood, use RT-PCR method to test the blood mononuclear cell surface expression of TLR-4 mRNA,use Elisa method to test the TLR-4 protein level in serum,serum NF-кB,pro-inflammatory factor IL-6,TNF-α and anti-inflammatory factor IL-4 protein level in serum. Results: (1) Immediately after 60%VO2max exercise,blood mononuclear cell surface TLR-4 mRNA expression and TLR-4 serum protein levels decreased,serum NF -кB,IL-4,TNF-α protein levels rise,levels of serum IL-6 protein rise first and then decreased;In addition to TLR-4mRNA expression,other indicators are return to th