抗独特型抗体6B11改善卵巢癌免疫抑制微环境的体外研究.doc

  Anti-idiotype (Id) antibody 6B11 amends the immunosuppressive state of the ovarian cancer microenvironment in vitro# 5 10 15 20 25 30 35 40 Liu Chanzhen, Chang Xiaohong, CuiHeng, Zhang Hong** (Gynecologic oncologic center of Peking University People's Hospital, Beijing 100044, China) Abstract: Objectives: The goal of this investigation was to study the immune regulation and potential therapeutic value of the ovarian cancer anti-idiotype (Id) antibody 6B11. Methods: Supernatants from cultured ovarian carcinoma cell were concentrated and normalized with selected cytokines. The normalized supernatants served as an in vitro experimental model of ovarian cancer immunosuppressive microenvironments. Results: After 6B11 or its anti-anti-Id antibody Ab3 was added, the numbers of CD4+CD25+ regulatory T cells, dendritic cells, and CD8+ T cells in the model were counted by flow cytometry. The results showed that normalized supernatants of SKOV3.IP cells (TGF-beta1 > 300 pg/ml; VEGF > 6 ng/ml) markedly increased the percentage of CD4+CD25+FOXP3+ regulatory T cells and CD8+ T lymphocytes that underwent apoptosis, while the differentiation and maturation of dendritic cells was inhibited. Treatment with 20 μg/ml of 6B11 or Ab3 decreased the number of CD4+CD25+ regulatory T cells. Moreover, the number of CD83+ mature dendritic cells increased in the presence of 6B11. Conclusion: This in vitro model may serve as an optional tool for studies of ovarian cancer immunosuppressive microenvironments. treatment with 6B11 in vitro amends the immunosuppressive state of the ovarian cancer microenvironment by impacting the percentage of CD4+CD25+ regulatory T cells and the differentiation of dendritic cells. Key words: anti-idiotype (Id) antibody, experimental model, immunosuppressive microenvironment 0 Introduction Ovarian cancer is the fifth leading cause of cancer deaths in women and the leading cause of death among gynecological cancers [1]. More effective adjuvant treatment modaliti