UV-B 辐射下悬沙对小球藻生长和DNA 损伤的影响.docVIP

2012-08-25  DOI: 10.3724/SP.J.1145.2012.00605  / 应用与环境生物学报 Chin J Appl Environ Biol 2012，18 (4): 605~609 UV-B辐射下悬沙对小球藻生长和DNA损伤的影响* 李 云1** 黄耀佳2 王晓蓉2 （1中国水产科学研究院东海水产研究所 上海 200090） （2华东师范大学生命科学学院 上海 200062）  摘 要  研究了UV-B辐射（17 μW cm-2，5 min/d）下不同浓度（0、100和1 000 mg L-1）和粒径组成（全粒径组和粒径 38 μm组）的悬沙对小球藻（Chlorella sp.）生长和 DNA损伤的影响. 结果显示，小球藻的生长可用Logistic增长模型拟合， 拟合后的生长参 数表明悬沙的“遮荫效 应”减轻了UV-B辐射对小球藻细胞的DNA损伤，从而对小球藻生长产生正 影 响. 悬沙浓度越高，UV-B辐射对小球藻细胞 DNA损伤程度越低，小球藻的环境负载能力a和瞬时增长率 K越大. 当悬沙 浓度为100 mg L -1时，全 粒径组a值和 K值小于粒径 38 μm组，且两组间小球藻细胞 DNA损伤差异显著（P 0.05），但 当悬沙浓度达1 000 mg L-1时，全粒径组a值和 K值大于粒径 38 μm组，两组间细胞的DNA损伤无显著差异（P 0.05）. 图3 表 2 参 24 关键词 悬沙；UV-B辐射；小球藻；细胞密度；DNA损伤 CLC Q949.2 : Q947.8 Effects of Suspended Solids on Growth and DNA Damage of Chlorella sp. Exposed to UV-B Radiation* LI Yun1**, HUANG Yaojia2 WANG Xiaorong2 (1East China Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Shanghai 200090, China) (2School of Life Sciences, East China Normal University, Shanghai 200062, China) Abstract Effects of suspended solids (SS) with different concentrations (0, 100 and 1 000 mg L -1) and different particle sizes (all size range and size 38 μm) on the growth and DNA damage of Chlorella sp. exposed to UV-B radiation (17 μW cm-2, 5 min/d) were studied. The growth of Chlorella sp. was well predicted by a logistic model, and the growth parameters showed that, SS attenuated DNA damage induced by UV-B and promoted the cell growth. With the increase of SS concentration, the cellular DNA damage induced by UV-B decreased, and the environmental carry capacity (a) and instantaneous rate of increase (K ) increased. When the cells were exposed to SS of 100 mg L -1, the values of a and K were lower in all-size-range group than in the group of size 38 μm, and a significant difference in the cellular DNA damage (P 0.05) appeared between them. When exposed to SS of 1 000 mg L -1, a and K were higher in all-size-range group than in the group of size 38 μm, but no significant d