安贞医院膜片钳.pdfVIP

吴坤 2013-1-23 2013-1-23 -11--2233 wwwwww..cciibbcchhiinnaa..ccoomm 中国第一家膜片钳仪器公司，集研发、代理、销售和维修为一体。目前北京、 上海、广州、武汉、成都、哈尔滨均有常驻销售人员和工程师，客户覆盖全国。 膜片钳系统 Patch Clamp System Patch Clamp System PPaattcchh CCllaammpp SSyysstteemm What is Patch Clamp? What is Patch Clamp? WWhhaatt iiss PPaattcchh CCllaammpp?? Patch-clamping is an electrophysiological technique in which we are able to CLAMP the VOLTAGE of an isolated piece of cell membrane (or whole-cell). By clamping the voltage we are able to observe CURRENTS that flow through ION CHANNELS. It is the current that the patch-clamp amplifier supplies to hold the voltage steady (clamped) that we measure. Patch-clamp recording allows us to measure very small currents - in the pA range. • Sakmann, Neher et al revolutionized the field of electrophysiology in 1981 with their paper “Improved patch-clamp techniques for high resolution current recording from cell and cell-free Pflügers Arch membrane patches” ( . 391, 391 339911 85-100). • In 1991, Neher and Sakmann were rewarded for their pioneering efforts in patch-clamp recording when they jointly won the Nobel Prize in Physiology or Nobel Prize NNoobbeell PPrriizzee Medicine. • Some companies have close relationship with Neher such as HEKA, Cytocentrics etc. :