鸭源呼肠孤病毒感染引起雏鸭免疫损伤的观察.pdfVIP

中国农业科学 2010,43(21):4514-4520 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2010.21.022 鸭源呼肠孤病毒感染引起雏鸭免疫损伤的观察 李 爽，谷长勤，白家媛，张 红，张万坡，程国富，胡薛英 （华中农业大学动物医学院，武汉 430070 ） 摘要：【目的】观察鸭源呼肠孤病毒对雏鸭脾、法氏囊的病理损伤及相关免疫指标，初步阐明鸭源呼肠孤病 毒对所引起的机体免疫器官损伤以及机体免疫抑制，为进一步探讨发病机理和免疫防治提供理论依据。【方法】7 日龄樱桃谷雏鸭人工感染鸭源呼肠孤病毒，分别观察脾、法氏囊组织病理学变化，检测脾、法氏囊指数，外周血 CD3+ + 、CD8 T 细胞阳性率，脾、法氏囊 IgY 生成细胞，并分析试验组及对照组差异显著性。【结果】感染鸭源呼肠 孤病毒后，雏鸭脾出现明显坏死灶，之后出现肉芽肿结构，法氏囊固有层淋巴滤泡明显减少；脾、法氏囊指数降 低；外周血 CD3+ + 、CD8 T 细胞阳性率降低；脾 IgY 生成细胞数量增多、法氏囊 IgY 生成细胞数量减少。【结论】鸭 源呼肠孤病毒感染可导致雏鸭免疫器官严重损伤并引起免疫抑制。 关键词：鸭源呼肠孤病毒；病理变化；免疫损伤；免疫抑制 Observation of Duckling Immune Injury Caused by Duck-Origined Reovirus Infection LI Shuang, GU Chang-qin, BAI Jia-yuan, ZHANG Hong, ZHANG Wan-po, CHENG Guo-fu, HU Xue-ying (College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070) Abstract: 【Objective 】 The pathological damages of spleen and bursa of Fabricius in ducklings caused by duck-deprived reovirus and related immune index were observed. The mechanisms of immune organ damage and immune suppression upon duck-derived reovirus infection were studied for providing a theoretical basis for further studies on pathogenesis and immune prevention and control. 【Method 】 Cherry Valley ducklings at 7-day old of age were artificially infected with duck-derived reovirus, + then histopathological changes of spleen and bursa of Fabricius were observed. Spleen and bursa index, the positive rate of CD3 , + CD8 T cells in peripheral blood ，and IgY-producing