灰毛豆树皮中的杀虫成分及其杀虫活性.pdfVIP

昆虫 学报ActaEntomologicaSinica，December2011，54(12)：1368—1376 ISSN0454-6296 Isolation andidentification ofinsecticidalcompounds from Tephrosiapurpurea (Fabaceae)barkand theirinsecticidalactivity LIYou—Zhi一．LIGuan—Hua WEIXiao—Yi， ， LIU Zhong．Hua ．XU Han—Hong’ (1．NationalResearchCenterofEngineeringTechnologyforUtilizationofBotanicalFunctionalIngredients，． HunanAgriculturalUniversity，Changsha410128，China；2．HunanProvincialKeyLaboratoryforBiologyand ControlofPlantDiseasesandInsectPests，CollegeofBio—SafetyScienceandTechnology，HunanAgriculturalUniversity， Changsha410128，China；3．KeyLaboratory ofNaturalPesticideandChemicalBiology，CollegeofNaturalResources andEnvironment，SouthChinaAgriculturalUniversity，Guangzhou510642，China；4．SouthChinaBotanicalGarden， ChineseAcademyofSciences，Guangzhou510650，China) Abstract：Inordertodetermineinsecticidalcompoundsfrom themethanolextractsofTephrosiapurpurea bark．theactivecompoundswereisolatedby activity—guided fractionationwith column chromatography andidentifiedbasedonNMR (nuclearmagneticresonance)andMS (massspectrometry)data．Slide dipmethodwasperformedtodeterminetheinsecticidalactivitiesofeachcompoundagainstMyzuspersicae adults．andtopicalapplicationwasconductedtodeterminecontacttoxicityofeachcompoundagainstthe 3rd instarlarvaeofPlutteUaxylostella．Ten knowncompoundswereisolated and identified．i．e．，12a hydroxyrotenone，4-hydroxyemoroidocarpan，pachyrrhizine，rotenone，6一methoxycoumarin，(一)一edunol， obovatin， pongachin． 12一acetyelliptinol and 2-hvdr0xvr0tenone． A11 these compounds exhibited insecticidalactivityagainstthe4thinstarlarvaeofAedesalbopictuswiththeLCnvaluebeing12．5，22．1，