铜绿微囊藻生长对培养液pH值和氮转化影响.pdfVIP

J. Lake Sci.(湖泊科学), 2009, 21(1): 123-127 . E-mail: jlakes@ ©2009 by Journal of Lake Sciences * 铜绿微囊藻生长对培养液 pH 值和氮转化的影响 吴 剑, 孔 倩, 杨柳燕**, 肖 琳, 孙 成 (污染控制与资源化研究国家重点实验室, 南京大学环境学院, 南京 210093) 摘 要: 在不同初始 pH 值条件下培养蓝藻水华优势种铜绿微囊藻(Microcystis aeruginosa), 研究其生长过程对培养液的 pH 值 和氮转化的影响. 结果表明, 处于延迟期和对数生长期的铜绿微囊藻使培养液的 pH 值上升, pH 从初始的 7.2、8.0 和 8.8 分别 上升到最大值 10.02、10.34 和 10.94; 稳定期和衰亡期的微囊藻使培养液 pH 值降低. 初始 pH 值为 8.8 时, 最终 pH 降至 8.66. 在微囊藻生长过程中, 培养液中 NO3-含量逐渐降低, NO2-含量先升高再降低, 分别在 60d 和 67d 后降至零. 试验结束时, 不同 pH 值培养液中总氮含量比起始时分别下降了 25.97% 、27.52%和 28.16%, 说明化合态无机氮可以经过反硝化作用生成氮气而 溢出培养液, 因此, 较大密度水华水体中存在反硝化的脱氮过程. 关键词: 铜绿微囊藻; pH 值; 氮转化 Effect of the growth of Microcystis aeruginosa on the pH value and the nitrogen transformation in the medium WU Jian, KONG Qian, YANG Liuyan, XIAO Lin SUN Cheng (State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093, P.R.China) Abstract : Microcystis aeruginosa, dominance specie in the cyanobacterial bloom, was cultured under the different initial pH values in order to study the effect of the growth of M. aeruginosa on the pH value and the nitrogen transformation in the medium. The results showed that the pH value rose in the lag phase and exponential phase of M. aeruginosa, and the maximum pH values rose from 7.2, 8.0 and 8.8 to 10.02, 10.34 and 10.94 respectively, then decreased in the stationary phase and the decline phase of M. aeruginosa. While initial pH value was 8.8, it decreased to 8.66 at the end of the experiment. NO3- concentration decreased and NO2- concentration rose earlier then decreased later in the medium during the growth period of M. aeru