产enterocinE5细菌素粪肠球菌发酵条件的优化.docVIP

产enterocin E5细菌素粪肠球菌发酵条件的优化 崔德凤1，周波1，杨桂梅1，李焕荣1，阮文科1汪明2张永红1 * (1.北京农学院 动物科技学院, 北京昌平102206；2.中国农业大学动物医学院, 北京100193) 摘要：　目的　对产enterocin E5细菌素粪肠球菌的发酵条件进行研究。方法　采用琼脂扩散法测定发酵上清液对大肠埃希菌K88的抑菌活性。结果　确定粪肠球菌E5产细菌素的发酵条件为MRS培养基，最适温度为37℃，最适起始pH值6.5，最佳接种量2%,种龄14 h，发酵时间16h,最佳培养基组分氮源为1%胰蛋白胨、0.5%酵母浸粉，最佳碳源为1%葡萄糖、0.5%蔗糖, 0.1% Tween-80有利于enterocin E5的产生。这是分离自北京优良商品猪黑六产enterocin E5粪肠球菌的首次报道。 关键词　enterocin E5；粪肠球菌；发酵条件；抗菌活性 Study on the optimum enterocin E5 production condition of Enterococcus faecalisE5 CUI Defeng1 ,Zhou bo YANG Guimei1 , LI Huanrong1 ,RUAN Wenke1 ,WANG Ming2 , ZHANG Yonghong1* ( 1. College of Animal Science and Veterinary Medicine, Beijing University of Agriculture, Beijing 102206, China ; 2.College of Veterinary Medicine , China Agricultural University , Beijing 100193 , China) Abstract　Objective　To investigate the fermentation conditions for growth and antibacterial substance production by Enterococcus faecalis E5.Method　The antibacterial activity of the supernatant against Escherichia coli K88 was tested with agar well diffusion. Results　The optimum fermentation conditions for E5 to produce enterocin E5 included the following: tryptone 1%, yeast extract 0.5%, glucose1%,sucrose 0.5%, and Tween-80 0. 1%.This formula of modified MRS could stimulate the generation of enterocin E5.The optimum initial pH was 6.5,while the best temperature was 37℃,with the inoculation amount of 2% (v/v), the seed age of 14 h,and the fermentation period of 16 h. This is the first report on enterocin E5 produced by Enterococcus faecalisE5 isolated from Beijing black six swine. 　 Key words　 enterocin E5; Enterococcus faecalis;Fermentation conditions; antibacterial activity 乳酸菌(Lactic acid bacteria)是一类能够发酵糖产生大量乳酸细菌的统称，肠球菌（enterococci）是乳酸菌中对营养要求不高、容易培养、分布广泛的一类革兰氏阳性球菌。长期以来在乳制品加工、蔬菜及肉制品的发酵工艺中广泛应用。肠球菌在生长繁殖过程中除产生乳酸、乙酸等有机酸外还能产生多种具有抑菌或杀菌活性的细菌素，在抑制多种动物源病原微生物和食物腐败菌等方面具有重要作用。 (Abriouel, Ben Omar 等， 2006)从食品中分离出产生enterocin P细菌素的肠球菌并对其特性进行了深入的研究[3]。分离自牛粪堆肥中的肠球菌显示出对食物源病原微生物和腐败菌的抑制活性[2]。(Siragusa, 1992)从牛的