昆布水提物对人子宫内膜癌细胞系BMDC1（ADR+-+）多药耐药性的影响.DOCVIP

临床医学论文-昆布水提物对人子宫内膜癌细胞系BMDC1（ADR+/+）多药耐药性的影响 ???????????? 作者：李巧敏，连易水，张静，单保恩，赵瑞撵，沈莉? 【摘要】? 目的：研究昆布提取物（TLPE）在体外对耐药细胞BMDC1（ADR+/+）的逆转作用，探讨其逆转肿瘤多药耐药的机制. 方法：应用流式细胞术测定不同剂量TLPE对BMDC1用药前后细胞内蛋白激酶Cα（PKCα）和谷胱甘肽转移酶π（GSTπ）的表达变化，测定细胞周期和凋亡率；应用姬姆萨染色技术观察细胞形态学改变及细胞凋亡情况；应用比色法检测各组细胞ATP酶（ATPase）变化. 结果：TLPE在70～300 mg/L浓度范围内能明显降低BMDC1细胞内PKCα和GSTπ的表达（P0.01），并将细胞阻滞于S期，但不诱导细胞凋亡；TLPE对细胞内ATPase无影响（P0.05）. 结论：TLPE对子宫内膜癌细胞系BMDC1（ADR+/+）具有耐药逆转作用，其逆转作用与其诱导PKCα和GSTπ的表达降低有关. 【关键词】? 昆布水提物；子宫内膜肿瘤；多药耐药 　　【Abstract】 AIM: To study the multidrug resistance (MDR)reversing effect of Chinese drug Thallus Laminariae PE (TLPE) on human endometrial cancer cell line BMDC1 （ADR+/+） in vitro and its underlying mechanism. METHODS: The expressions of PKCα and GSTπ at the level of protein in BMDC1 cell treated with different doses of TLPE were analyzed by flow cytometry. The cell cycle and apoptosis rate of BMDC1 （ADR+/+） cells treated by TLPE were detected. Their morphological changes were observed by Gimsa staining methods. Colorimetry was used to detect the changes of ATPase on BMDC1 （ADR+/+） cells treated by TLPE. RESULTS: After interfered by TLPE (70-300 mg/L), the expressions of PKCα and GSTπ in cells were decreased significantly （P0.01）. TLPE could block cell cycle at S period, but it did not obviously induce cell apoptosis. There was no significant change in the expression of ATPase on TLPEtreated cells. CONCLUSION: TLPE can reverse MDR of BMDC1 （ADR+/+） cell, and the mechanism may be related to the decreasing expressions of PKCα and GSTπ in cells. 　　【Keywords】? TLPE; endometrial neoplasms; multidarng resistance 　　0引言 　　多药耐药(multidrug resistance, MDR)的发生与多种因素有关，信号转导通路，GSTπ和细胞ATP酶（ATPase）在肿瘤细胞表达与其功能发挥可能起到关键的调控作用［1］. 昆布作为传统中药，常参与抗癌中药的配伍. 昆布提取物（thallus laminariae PE, TLPE）对多种肿瘤细胞株有抑制作用，其机制主要是抑制肿瘤细胞的增殖和(或)杀死肿瘤细胞［2］. 除此之外，TLPE还可通过降低耐药细胞Pgp的表达，从而达到逆转多药耐药的作用［3］. 目前，有关昆布逆转肿瘤细胞多要耐药的作用机制还少见报道. 我们通过TLPE 对信号传导分子PKCα，GSTπ及细胞ATPase影响的体外实验研究，探讨昆布对逆转子宫内膜癌多药耐药作用的相关机制. 　　1材料和方法 　　1.1材料