6株土壤拮抗放线菌的抗菌活性测定及初步鉴定.pdfVIP

PlantDiseasesandPests 2011，2(1)：53-55，66 Determination of Fungicidal Activities of S、1；X^ Antagonistic Actinomycetes in SoiI and Their Preliminary Identification LONG Jian-you，XIA Jian-rong CollegeofEnvironmentalScienceandEngineering，GuangzhouUniversity，GuangdongProvince，Gunagzhou510006，China Abstract [Objective]Thepaperwastopreliminarilyidentifythespeciesofsixactinomycetesstrains，andtodeterminethefungicidalactivitiesoftheirfermenta- tionproducts． M『ethod1111especiesofsixactinomycetessrtainswereidentifiedbymorphologicalmethod，nadtllefungicidalactivitiesofthefemrentationproducts 0fs actinomycetes strainsweresystematicallydeterminedbyseriesofmehtodsincludingmycelial growthrateinhibitionmehtod，sporegerminationmethod ，potting andfieldtest． 『Result1MorphologicalidentificationresultsshowedthatsixsrtainsbelongedtoStreptomyces．Biologicaldeterminationresultsindicarde thathtein— hibifionrates ofhtefemrentationproductsofsixactinomycetesstrainswiththeconcentrationof500 IIll~,：,ainsthtemycehal growthofFusar／umaxysporum sp． Vasinfectum were allgreaterhtna 90％ ；hteinhihifionratesagainsthtemycelialgrowthofBotrytiscinereaPers．，A／ternar／attlterna~ nadFusarium oxysporumwere 81sogreaterthal180％． eird1南itionratesofhtefemr entationproductsofGZ-204nadGZ-331strains~ instthesporegerminationofBipolari~sorokinianaand Cercosporasorghiwere97．8％ ，98．2％ ，99．5％ and94．6％ ，respectively．Pottingtestshowedthattlleprotectioneffectsof thefermenattionproductsofGZ-2O4 andGZ-331strainsonwheatpowderymildew(Erysiphegraminis)were78．8％ and87．1％ ，andtheircureeffectswere62．4％ nad68．5％ ，respectively．Field testshowde thatthecontroleffectsof20Otimesfermenattionliquidsof GZ-2O4andGZ-331strainsonwhaetpowdery mildewwere50．5％ and69．2％ ．respective- lY． 『Condusion]Theresearchprovidde htereferencef0lrtlledevelopmentofnewpesticideswitllactinomycetesasthel~SOUlrCe． Keywords Actinomycetes：Screening；Fungicidalactivity；Binassay Intheresearch anddevelopmentofagriculturalantibiotics， porum f-cucumerinu