更年期大鼠Th1-Th2平衡与脾细胞凋亡.DOCVIP

临床医学论文-更年期大鼠Th1/Th2平衡与脾细胞凋亡 ??????????????? 作者：蒋竞，张绍芬，邹世恩，夏贤，陈王旬，刘瑞梓 【关键词】? 更年期 ??? 摘要：目的? 了解更年期大鼠脾细胞凋亡与辅助性T细胞1(Thelper l, Th1)和辅助性T细胞2(Thelper 2, Th2)型细胞因子的增龄性改变。方法? 应用Annexin VFITC/PI双染色流式细胞仪测定脾细胞凋亡率； ELISA法测血清γ干扰素(interferonγ, IFNγ)、白介素4(interleukin4, IL4)水平。结果? 更年期组大鼠脾细胞凋亡率(10.3% vs. 7.7%, P0.05)、血清IFNγ质量浓度明显高于年轻组大鼠(23.80ng/L vs. 10.92ng/L，P0.01)；更年期组大鼠与年轻组相比血清IL4质量浓度有下降趋势，但差别无显著意义(0.1296ng/L vs. 0.3094ng/L, P0.05)；更年期组血清IFNγ/IL4比值明显高于年轻组(183.64 vs. 35.29，P0.01)，Th1/Th2平衡朝Th1漂移。结论? 大鼠衰老过程中Th1/Th2平衡朝Th1漂移，脾细胞凋亡增加与IFNγ水平增高有关。?　　关键词：更年期；脾细胞；凋亡；Th1；Th2 　　The changes of Th1/Th2 balance and apoptosis in splenocyte of menopausal rats 　　ABSTRACT: Objective? To investigate the relation between the apoptosis in splenocyte and the changes of Th1/Th2 balance in serum of menopausal rats. Methods?Twenty female SD rats were divided into two groups: menopausal group(n=10;15 months), young group (n=10; 5.5 months). The apoptosis in splenocyte of rats was measured by Annexin VFITC/PI flow cytometric assay. The levels of IFNγ and IL4 in serum were measured by ELISA. Results? The percentages of apoptosis cells in splenocyte of menopausal rats were significantly higher than that of young rats. The serum IFNγ level of menopausal rats was significantly higher than that of young rats(23.80ng/L vs. 10.92ng/L, P0.01). The level of IL4 was similar in menopausal and young groups. Conclusion? The increasing of apoptosis in splenocyte of menopausal rats is related to the increasing of IFNγ levels with aging. 　　KEY WORDS: menopause; splenocyte; apoptosis; Th1; Th2 　　人体免疫功能随增龄而衰退是衰老过程中的一个关键因素，以致对感染及某些自身免疫性疾病易感。资料显示衰老时免疫功能的变化与免疫细胞凋亡密切相关[1]。近年研究发现[2]，老年人外周血CD+4T细胞产生γ干扰素(cinterferon γ, IFNγ)高于年轻组，而产生白介素4(interleukin4, IL4)降低，提示老年个体存在Th1极化现象。目前有关细胞因子变化与免疫细胞凋亡之间关系的研究较少。有研究表明活化诱导的淋巴细胞凋亡(activationinduced cell death, AICD)在免疫应答的负调节中发挥重要作用，而细胞因子能影响AICD[3]。我们通过检测脾细胞凋亡率，测定血清IFNγ、IL4和计算IFNγ/IL4比值，来探讨更年期大鼠脾细胞凋亡的变化及Th1、Th2型细胞因子的改变，为深入研究更年期随年龄增长而发生的免疫功能变化提供实验依据。 　　1? 材料与方法 　　1.1? 实验动物? 采用清洁级SD雌性大鼠(由本校上海