重组人肝再生增强因子对梗阻性黄疸大鼠肝细胞线粒体功能的保护作用.DOCVIP

临床医学论文-重组人肝再生增强因子对梗阻性黄疸大鼠肝细胞线粒体功能的保护作用 ????????????? 作者：唐春 别平 李昆 张玉君 马正伟? 【摘要】? 目的 探讨重组人肝再生增强因子(rhALR)对梗阻性黄疸大鼠肝细胞线粒体功能及肝功能的保护作用。方法 144只健康Wistar大鼠随机分为SHAM组、BDORBF组、BDORBFrhALR组，分别检测其线粒体功能及肝功能的变化情况，电镜观察各组大鼠肝细胞线粒体的形态学改变。结果 胆道梗阻后，大鼠线粒体功能及肝功能均出现明显损伤(P＜0.05或P＜0.01)，BDORBFrhALR组肝细胞线粒体功能及肝功能的损害程度明显轻于BDORBF组(P＜0.05)，并且当胆管再通后，其恢复更快。电镜观察梗阻性黄疸后，肝细胞线粒体出现明显损伤，BDORBFrhALR组病理改变明显轻于BDORBF组。结论 rhALR对梗阻性黄疸大鼠肝细胞线粒体功能及肝功能具有明显的保护作用。 【关键词】? 重组人肝再生增强因子 线粒体功能 梗阻性黄疸 大鼠 ????? 【Abstract】? Objective? To study the beneficial effects of recombinant human augmenter of liver regeneration (rhALR) on mitochondrial function and liver function in rats with obstructive jaundice. Methods?A total of 144 healthy Wistar rats were randomly divided into three groups, ie, sham operation group (Group A), obstructive jaundice and biliary tract recanalization (Group B) and obstructive jaundice and biliary tract recanalization plus rhALR (Group C) to respectively observe, at selected time points, the changes of the liver and mitochondrial function as well as morphologic changes of the mitochondriae by electroscope. Results? There occurred more severe damage to liver function and mitochondrial function in Group B, compared with Group A (P＜0.05 or P＜0.01). Compared with Group B, the degree of damage to mitochondrial and liver function was lighter in Group C (P0.05). The electron microscope showed that mitochondrial structure was damaged in obstructive jaundice rats. The pathological change in Group C was significantly less than that in Group B. Conclusion?rhALR can exert obvious protective effect on mitochondrial function and liver function of rats with obstructive jaundice. ??? 【Key words】? Recombinant human augmenter of liver regeneration; Mitochondrial function; Obstructive jaundice; Rats ??? 线粒体是梗阻性黄疸时肝细胞内最先受损害的细胞器［1］。线粒体功能是决定肝细胞功能的重要因素［2］。本实验通过给予梗阻性黄疸大鼠以重组人肝再生增强因子 (recombinant human augmenter ofliver regeneration，rhALR)，旨在了解rhALR能否通过保护及改善肝细胞线粒体功能、肝功能，为梗阻性黄疸的围手术期处理提供帮助。 ??? 1? 材料与方法 ??? 1.1? 实验动物模型制