合作猪SLA_DQA基因外显子2多态性分析_张国华.doc

·研究报告·  生物技术通报 BIOTECHNOLOGY BULLETIN  2014年第4期  合作猪 SLA-DQA 基因外显子 2 多态性分析 张国华1 马小军1，2 吕伟丽1 （1. 甘肃农业大学动物医学院，兰州 730037 ；2. 甘肃省草食动物生物技术重点实验室，兰州 730070）  摘  要 ： 旨在研究合作猪 DQA 基因外显子 2 多态性，确定其等位基因数、核苷酸多态位点、氨基酸多态位点及各个等位 基因之间的遗传关系，分析其进化意义。选用 PCR-SSCP 对 439 只合作猪 SLA-DQA 基因外显子 2 的多态性进行检测 ；测序群体内 因变异而产生的各等位基因序列，并分析序列数据。结果显示，在合作猪 SLA-DQA 外显子 2 中发现了 7 个新等位基因，共 18 个 核苷酸多态位点，10 个氨基酸多态位点。合作猪 SLA-DQA 外显子 2 具有较丰富的多态性，群体内可能蕴藏着更加丰富的遗传资 源 ；合作猪 SLA-DQA 外显子 2 基因最初可能由一个等位基因突变分化成一大类基因 ；合作猪 SLA-DQA 外显子 2 序列与各个猪种 的 SLA-DQA 外显子 2 序列具有较高的同源性，预示着这些猪种的 SLA-DQA 外显子 2 基因最早可能来源于其分歧之前的共同祖先 原始序列 ；新发现的 7 个 SLA-DQA 外显子 2 等位基因，可能由遗传关系较近的两个等位基因突变产生。 关键词 ： 合作猪 SLA-DQA 外显子 2 多态性 PCR-SSCP  Analyzing Polymorphism of SLA-DQA Gene Exon 2 in the Hezuopig （1. College of Veterinary Medicine，Gansu Agricultural University，Lanzhou 730037 ；2. Gansu Key Laboratory of Herbivorpous Animal Abstract: The polymorphism, allele number, nucleotide polymorphism sites, amino acid polymorphism sites, the genetic relationship and evolutionary significance of the alleles of the SLA-DQA gene exon 2 in Hezuo pigs were studied. Variation of SLA-DQA gene in the 439 Hezuopigwas investigated by amplification of exon 2 using PCR, followed by single-strand conformational polymorphism（SSCP）, DNA sequencing and sequence data analysis. Result showed that seven alleles were found in the SLA-DQA geneexon 2. Eighteen nucleotide polymorphism sites and ten amino acid polymorphism sites were identified in seven SLA-DQA gene exon 2 haplotypes. The study indicated that a high level of sequence polymorphism existed in SLA-DQA gene exon 2 of Hezuo pigs, and the population might have more genetic resources. SLA-DQA gene exon 2 in Hezuo pigs might be differentiated into two major categories alleles from one mutant alleles at first. SLA-DQA gene exon 2 in Hezuo pigs and other pig had high homology, which indicated that SLA-DQA gene exon 2 in pig might come from primordial sequences that were present in a common ancestor and persisted in the pig