四种葡萄病毒外壳蛋白基因的系统进化研究.pdfVIP

四种葡萄病毒外壳蛋白基因的系统进化研究 王建辉1，刘建军扣，陈克玲1，李洪雯1，何建1，关斌1 (1四川省农业科学院同艺研究所，成都610066；2四川省农业科学院，成都610066) 摘要：葡萄是一种在全世界栽培面积大、产量高的重要果树。葡萄可以被多种病毒感染，分别诱 分离株的全长印进行多重比对分析。根据每种病毒的不同分离株的全长印多重比对同一性值人小， 性高达99．9-92．5％，也许是一类单独、不分化的种群。进行葡萄病毒的结构蛋白基因系统进化研究， 为以后利HJ病毒诱导基因沉默开展葡萄抗病育种打下理论基础。 StudiesonCPGenesofFour Viruses Phylogenetic Grapevine WANG GUANBinl Jian—huil，LIU Hongwen‘，HEJianl，and Jian-jun“，CHENKe—lin91，LI Institute,Sichuanof and 61 (1Horticulture AcademyAgricultureScience,Chengdu and 61 AcademyofAgricultureScience,Chengdu0066,China) with around isoneofthemost economichorticulture Abstract：Grapevine important crops highyields theworld．Sofartherearemoreandmorevirusesthathavebeenfoundtoinfectthe withdifferent grapevine leafdiseaseetc．Onetable leafroll wood andfan disease，rugosecomplex grapevariety， symptoms，including infectedwithfourdistinctofviruses DAS—ELISA，including Fujiminofi，Was using GVAand hadbeenconfirmedRT-PCR．Basedon GVB，which homologymulti—alignment by cpgenes by ofeachviruscollectedfrom wasseemedtocluster withtheknownisolates Genebank,it comparing analysis 16 ofGLRaV一2isolates distinct cluster16 ofGVAisolatesintotwo cpgenes intothree groups，to cpgenes cluster7 ofGVBisolatesintothree ofGLRaV一3 distinguishedgroups，to cpgenes groups．Thehighidentity