糖皮质激素受体与类风湿关节炎证型相关性研究.pdfVIP

第16 卷 第1 期 2010 年1 月 Vol.16 No.1 Guiding Journal of Traditional Chinese Medicine and Pharmacy January.2010 糖皮质激素受体与类风湿关节炎证型相关性研究 王康惠，吴启富，康信忠，丁朝霞，鲁 莹 （南方医科大学中医药学院，广东 广州 510515） [摘要] 目的：探讨类风湿关节炎患者外周血单个核细胞（PBMC）中糖皮质激素受体（GR）亚型α和β 的蛋白质表达水平 与中医证型的相关性。方法：选取符合纳入标准的RA 患者60例，其中湿热痹阻证组与瘀血痹阻证组各30例，再选取20名健康 人作为对照组。采用蛋白印迹（WB）检测外周血PBMC 中GRα和GRβ蛋白质水平。结果：GRα和GRβ蛋白质水平: （1）湿热痹阻证 组和瘀血痹阻证组与对照组比较均偏高，差异有统计学意义(P＜0.05) ；（2）湿热痹阻证组高于瘀血痹阻证组(P＜0.05) 。结论： GRα和GRβ蛋白质水平与RA 中医证型具有相关性。 [关键词] 类风湿关节炎；中医证型；GRα ；GRβ [ 中图分类号] R241.4 [文献标识码] A [文章编号] 1672-951X （2010）01-0010-03 Correlativity between Glucocorticoid Receptor and Chinese Medicine Syndrome in Rheumatoid Arthritis WANG Kang -Hui, WU Qi-Fu, KANG Xin-Zhong, DING Zhao-Xia,LU Ying Southern Medical University ，Guangzhou ，China ，510515 [Abstract] Objective ：To investigate the relationship between the expression of GRα and GRβ protein in the peripheral blood mononuclear cell(PBMC) of RA patients and Chinese Medicine Syndrome. Methods ：Sixty RA patients meeting the inclu－ sive criteria were differentiated into dampness-heat blockage type(30 cases), stasis accumulation type(30 cases). The expression of GRα and GRβ protein in PBMC was detected by western blotting technique in RA patients and healthy people(20 cases). Result ：The expression of GRα and GRβ protein in RA were higher than that of control group(P0.05). Dampness-heat block－ age type of RA was higher than that of control group(P0.05), so as stasis accumulation type(P0.05). Dampness-heat block－ age type of RA was higher than that of stasis accumulation type of RA(P 0.05). Conclusion：The expression of GRαand GRβprotein in the PBMC of RA patients is related to Chinese Medicine Syndrome.