低聚木糖的免疫调节作用(台湾)2012(18_195).pdfVIP

Food Sci. Technol. Res., 18 (2), 195–199, 2012 Immunomodulatory Effects of Xylooligosaccharides 1* 2 1 3 Hua Han cHen , Yu Kuo cHen , Hung Chia cHang and Su Yi Lin 1 Departme nt of Food Science, National Penghu University of Science and Technology, Penghu 880, Taiwan 2 Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ 08854 3 Department of Applied Science of Living, Chinese Culture University, Taipei, Taiwan Received October 13, 2011; Accepted November 15, 2011 Xylooligosaccharides (XOs) are considered as food ingredients, and exhibit the prebiotic effects related to gut modulation. However, no related research is available to explain their immunomodulatory effects. This report elucidated their immunomodulatory effects through the variations of proinflammatory me- diators in vitro. We found that XOs (0.1−100 μg/mL) induced tumor necrosis factor alpha (TNF-α), IL- 1β, IL-6 and nitric oxide (NO) production in un-stimulated macrophages, RAW264.7 cells. Furthermore, pre- and post-treated XOs (0.1 −100 μg/mL) dose-dependently suppressed TNF-α, IL-1β, IL-6 and NO production and induced IL-10 production in lipopolysaccharide (LPS)-stimulated RAW264.7 cells with- out exerting cytotoxicity. Of note is that prostaglandin E (PGE ) production didn’t change significantly 2 2 through the XOs treatment. These data demonstrate that XOs potently down-regulates the LPS-induced inflammatory response. The in vitro assessment of inflammatory mediators should be useful in further characterization of the effects of XOs on imm