SFE_CO_2和超声法提取丹参药渣的对比研究.pdfVIP

2008年 12 月第15卷第12期 中国中医药信息杂志 ·59· ·制剂与工艺 · SFE-CO 和超声法提取丹参药渣的对比研究 2 1 1 1 2 2 张 明 ,刘汉清 ,韩晓珂 ,马俊华 ,马继永 (1.南京中医药大学,江苏 南京 210046；2.山东华信制药有限公司,山东 济南 274009) 摘要：目的 探讨不同提取工艺提取丹参药渣中丹参酮ⅡA成分含量,以便对丹参药渣综合利用。方法 采用超 临界二氧化碳(SFE-CO )萃取丹参酮ⅡA,并与超声提取法相比较。结果 SFE-CO 萃取的最佳工艺为萃取压力35 MPa, 2 2 夹带剂乙醇用量为 100%,萃取温度 40 ℃,萃取时间 2 h,含量高达 3.87 mg；而超声提取法只有 2.89 mg。结论 用 SFE-CO2 萃取丹参药渣中丹参酮ⅡA,其提取率及纯度均高于超声提取法,且丹参药渣可以综合利用。 关键词：水提丹参药渣；超临界二氧化碳；丹参酮ⅡA；提取工艺；正交设计 中图分类号：R283.5 文献标识码：A 文章编号：1005-5304(2008)12-0059-02 ZHANG Ming, LIU Han-qing, HAN Xiao-ke, et al (Nanjing University of TCM, Nanjing 210046, China) To study the different extraction methods for the content of tanshinone ⅡA in water decocted Radix Salviae Miltiorrhiza, in order to develop comprehensive utilization of Salvia miltiorrhiza. Tanshinone ⅡA was extracted by supercritical carbon dioxide fluid and compared with ultrasonic extraction. When the extraction pressure was 35 MPa, extraction temperature was 40 ℃, extraction time was 2 h and entrainer (ethanol) was 100 %, the yields of the tanshinone ⅡA was up to 3.87 mg, while the ultrasonic extraction was 2.89 mg. Tanshinone ⅡA extracted by supercritical carbon dioxide fluid was better than ultrasonic extraction and had higher purity. The comprehensive utilization of Salvia miltiorrhiza is available. water decocted Radix Salviae Miltiorrhiza ；SFE-CO2 ；tanshinone ⅡA；extraction method； orthogonal design 丹参为唇形科植物丹参 Salvia miltiorrhiza Bge.的干 品检定所,甲醇为色谱纯,水为重蒸水,其余化学试剂为分析纯, 燥根及根茎,味苦,性微寒,具有祛瘀止痛、活血通经、清心除 大生产水提丹参药渣及丹参药材购自亳州。 烦等功效。丹参的有效成分主要分脂溶性和水溶性两类,水溶