南苦参的化学成分研究论文.pdfVIP

Flavonoidcompoundsfromlitchi(LitchichinensisSonn.)seedsand appraisaloftheirα-glucosidaseinhibitoryactivities(Ⅱ) ShenRen1,2DuoduoXu1 ZhiPan1 RuizhiJiang YangGao YutingMa QipinGao*1 1ResearchandDevelopmentCenter,ChangchunUniversityofTraditionalChineseMedicine, Changchun130117,China2CollegeofTraditionalChineseMedicine,JilinAgriculturalUniversity, Changchunl30118,China. Abstract:Usingabioactivity-guidedmethod,twonewflavanoneglycosidesandeightpreviously reportedflavonoidswereisolatedfromanaqueousethanol(50％)extractoflitchi(Litchichinensis Sonn.)seeds.Byspectroscopicmethods,including1Dand2DNMR (heteronuclearmultiple quantumcoherence(HMQC),heteronuclearmultiplebondcorrelation(HMBC))techniquesand massspectrometry,thechemicalstructureofthenewcompoundswereelucidatedas (2R)-pinocembrin-7-O-[(2, 6-di-O-α-L- rhamnopyranosyl)-β-D-glucopyranoside] (1), pinocembrin-7-O-(6-O-α-L-arabinose-β-D-glucopyranoside)(2),andtheeightknowncompounds were determined to be quercetin (3), phlorhizin (4), pinocembrin-7-O-glucoside (5), kaempferol-7-O-β-D- glucopyranoside(6), onychin (7), nairutin (8), narcissin (9) and pinocembrin-7-O-[(6-O-β-D-glucopyranoside)-β-D-glucopyranoside)(10).Someoftheisolated flavonoidsweretestedfortheirinhibitoryeffectsonα-glucosidase;compounds3and4showed strongerinhibitionthanthecontrol,acarbose. Keywords:litchiseeds;flavonoidcompounds;α-glucosidaseinhibitoryactivity 南苦参的化学成分研究 叶纯 韩娜 薛睿 殷军 沈阳药科大学 中药学院，辽宁 沈阳 110016 摘要 ：南苦参(Streptocaulonjuentas)为萝藦科马莲鞍属植物。本文主要对南苦参根75％乙醇 提取物的大孔树脂柱60％乙醇洗脱物进行化学成分研究。采用硅胶柱层析、ODS反相柱层析， 制备液相 ，重结 晶等方法分离得到7个强心苷类化合物，通过核磁共振波谱法确定其化学结 构，分别为：Acovenosigeninβ-D-glucoside(1)，Digitoxigenin(2)，Digitoxigeninsophoroside(3)， Digitoxigeningentiobioside(4),Periplogenin(5),CorchorusosideC(6),Periplogeninglucoside(7) 和Biondianos