青花菜花球中芥子油苷多样性研究论文.pdfVIP

青花菜花球中芥子油苷多样性研究 王建升，虞慧芳，盛小光，赵振卿，张晓辉，顾宏辉 (浙江省农业科学院蔬菜研究所，杭州310021) 摘 要：以142份青花菜种质为材料，采用高效液相色谱法(HPLC)检测了青花菜花球中芥子油苷组分 和含量，并将所测青花菜材料主要分为4种类型．包括以2．羟基-3-丁烯基芥子油苷为主的侧链高修饰型、以 萝卜硫苷为主的高抗癌型以及可能受到特异转录调控因子调控的低脂肪类芥子油苷型和低吲哚类芥子油苷 型。其中，DH2和DH4为高抗癌型青花菜。DH2中萝卜硫苷和总芥子油苷的含量都明显高于商品种‘优 秀’，而DH4中萝卜硫苷的含量和比例都很高。两者可能是选育抗癌青花菜品种的好材料。 关键词：青花菜；芥子油苷；高效液相色谱(HPLC) VariationofGlucosinolatesinBroccoliHead(Brassica oleraceavar．italica) WANG Xiao-hui。GU Jian·sheng，YUHui—fang，SHENGXiao-guang。ZHENGZhen·qing，ZHANG Hong·hui。 (Institute 310021。China) ofVegetable，ZhejiangAcademyofAgriculturalSciences．Hangzhou levelsinedibleweredetected in Abstract：Glucosinolate HPLCdoubled and part by haploids commercial ofbroccoli(Brassicaoleraceavar．italica)fromfields，in of hybrids HangzhouZhejiang withtheresearchof and of in Province．Combining biosynthesisregulationglucosinolatesArabidop- sisandanti·cancercharacterofeach of inbroccoli glucosinolate，fourtypicalprofilesglucosinolates cultivarswerefoundand intofour modified separated types，includingaliphaticglucosinolatestype inwhichthe isthe in secondoneis progoitrinpredominatecomponentaliphatic glucosinolate，the anti-cancerinwhichthemost is withorwithouta little type aliphaticglucosinolateglucoraphanin orothermodified othertwo withlowlevelof progoitrin aliphaticglucosinolate．Thetypes aliphatic