少核年桔的辐射育种研究初报论文.pdfVIP

306 1 2 510640 , 516800 60 Co- 4 6 8Kr 68.4% 41.3% 16.5% 93.2% 5 3.3-7.0 17.6 45.5%-85.1% 11.8%-25.8% 97.3% 54.1% Studies on the Mutation Breeding for Few-Seeded Nianju Mutants 1 1 1 1 1 2 2 Tang Xiaolang , Ma Peipia ,Wu Wen , Huang Yongjing , Wang Ping , Huang Chiwen , Xiao manhong , 2 Chen Guotian 1. Institute of Fruit Tree Research of Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong; 2. Longmen County Agricultural Bureau, Longmen 516800, Guangdong Abstract: Mature autumn shoots were taken from the adult seedy Nianju ( Citrus reticulata Blanco) trees and the leaves of the shoots were stripped. The shoots were irradiated with gamma rays from 60Co at the doses of 0,4,6 and 8 Kr and dose rate of 230 r/min in January of 2005. All the treated buds were single-bud-grafted on Hong Ning Meng (C. limonia Osbeck) rootstocks after irradiation. The survivor rates were 93.2%, 68.4%,41.3% and 16.5% for the 0, 4,6 and 8 Kr treatment respectively. The plantlets were grown in the orchard in May of 2006.Through fruit investigation for 2 years, 5 few-seeded mutants were screened.The seed contents of the 5 few-seded mutants were 3.3-7.0 seeds/fruit, and the control was 17.6 seeds/fruit. The pollen alive rates of the 5 few-seded mutants were 45.5%-85.1%, and pollen germination rates were 11.8%-25.8%, which were obviously lower than 97.3% and 54.1% of the control respectively. One of the main causes producing few-seeded fruit was higher rate of pollen abo