分子排阻色谱法测定玻璃酸钠相对分子质量及其分布.pdfVIP

232 食品与药品 Food and Drug 2010年第 12卷第07期 分子排阻色谱法测定玻璃酸钠相对分子质量及其分布 王海英，郭学平，栾贻宏，马秋慧 （山东福瑞达生物医药有限公司，山东 济南 250101） 摘 要：目的 使用分子排阻色谱 （SEC ）法测定玻璃酸钠 （SH ）相对分子质量 （M ）及其分布。方法 考察进样浓度、柱 r 温及流速对SEC法测定SH 的Mr及其分布的影响。结果 进样浓度对高分子SH 的色谱峰形及测定结果影响较大，降低进样浓 度色谱峰形明显改善，且测定结果精密度 良好；柱温对SH 的M 测定结果有一定的影响；流速对SH 的M 及其分布基本无影 r r 响。结论 在SH进样浓度0.1 mg/mL ，柱温30 ℃，流速0.5 mL/min 的色谱条件下，用SEC法测定SH 的Mr及其分布的色谱峰 形良好，结果准确可靠，可用于SH 的质量控制和应用研究。 关键词：分子排阻色谱法；玻璃酸钠；相对分子质量 中图分类号：TQ460.7 文献标识码：A 文章编号：1672-979X(2010)07-0232-04 Determination of Relative Molecular Mass and Its Distribution of Sodium Hyaluronate by Size Exclusion Chromatography WANG Hai-ying, GUO Xue-ping, LUAN Yi-hong, MA Qiu-hui (Shandong Freda Biop harm Co., Ltd., Jinan 250101, China) Abstract: Objective To determine the relative molecular mass (Mr) and its distribution of sodium hyaluronate (SH) by the size exclusion chromatography (SEC). Methods The influences of sample concentration, column temperature and flow rate on the determination of M and its distribution by SEC were studied. Results For SH samples with r high molecular weight, the concentration had significant influence on the determination of Mr and its distribution by SEC. Good peak shapes and determination results could be obtained by decreasing the concentration of SH. Column temperature had some influence on the determination of Mr and its distribution by SEC, but flow rate had no influence. Conclusion The sample co