双葛解酲丸对酒精性脂肪肝大鼠炎症及脂质代谢的影响.docVIP

双葛解酲丸对酒精性脂肪肝大鼠炎症及脂质代谢的影响 魏文茂 朱丽丽 罗华丽 蒲晓东 (400016 重庆，重庆医科大学，中医药学院) 【摘要】 目的：探讨双葛解酲丸对酒精性脂肪肝模型的治疗效果。方法：将65只SD大鼠随机分为正常组（10只）和酒精性脂肪肝（AFL）组（55只），后者给予白酒灌胃建立AFL模型。8周后，处死其中5只（正常组1只，AFL组4只）病理组织学检查以验证其造模成功,然后将AFL组进一步分为模型对照组、双葛解酲丸高剂量组、双葛解酲丸中剂量、双葛解酲丸低剂量组和海王金樽治疗组。治疗4周末处死所以大鼠，检测血清大鼠的血清肿瘤坏死因子-aα)、白介素-6 (μmol/l)]和LEP[1.89±0.21（ng/ml）]明显低于模型对照组与海王金樽治疗组(p0.05),血清ADP[3.05±0.27（mg/ml）]明显高于模型对照组[2.17±0.37（mg/ml）]与海王金樽治疗组[2.69±0.32（mg/ml）(p0.05)]，双葛解酲丸对大鼠脂肪肝变及炎症明显改善。结论：双葛解酲丸对酒精性脂肪肝有治疗作用。 【关键词】酒精性脂肪肝，肿瘤坏死因子-a白介素-6575.5 ［文献标识码］ A Effects of Shuangge Jiecheng Pill of the inflammatory factors and lipid metabolism on alcoholic fatty liver rats Wei WenMao. Zhu LiLi .Luo HuaLi .Pu XiaoDong（School of Chinese Medicine , Chongqing Medical Uni versity , Chongqing 400050 , China) [ABSTRACT] Objective：Observe the effects of Shuangge Jiecheng Pill on GSH-PX, SOD ,FFAand MDA of liver tissue in rats of Alcoholic fatty liver, and approach the mechanism on the lipid peroxidation. Methods：The model of rats of Alcoholic fatty liver disease induced by 56°Hongxing wine by intragastric administration was used．60 cases were randomly divided into 6 groups, 10 were control group, 10 model group, 10 Haiwang(HW) group and 30 Shuangge Jiecheng Pill(SG) group with high dose(HD), middle dose(MD) and low dose(LD) on average. SG group were treated with different dosages of Shuangge Jiecheng Pill(20g、10g、5g crude drug/ kg·d) and compared with HW group administrated by Haiwang Jinzun Tablet（1.5g/kgbw）for 4 weeks. The changes of TNF-a, IL-6, LEP ,ADP and FFA of the rats in the different experimental groups were observed. Results: Compared with the control group, ADP in the model group was obviously decreased（P＜0.05) and IL-6,TNF-6,FFA and LEP was obviously increased（P＜0.05). Compared with the model group, ADP in the HW and SG group of HD , MD and LD were obviously increased（P＜0.05) while MDA and IL-6,TNF-6,FFA and LEP obviously decreased（P＜0.05). ADP in SG group of HD were obviously increased（P＜0.01，P＜0.05) while IL