烟草根系土壤微物肪酸生态学特性的研究.docVIP

烟草根系土壤微生物脂肪酸生态学特性的研究 郑雪芳，刘波(，蓝江林，朱育菁，车建美，苏明星 （福建省农业科学院农业生物资源研究所，福州 350003） 摘 要：为了探讨无致病力青枯雷尔氏菌对烟草根系土壤微生物群落结构的影响，本研究利用PLFA法测定了施用无致病力青枯雷尔氏菌和清水对照的烟草根系土壤，比较分析无致病力青枯雷尔氏菌处理和对照处理在对烟草根系土壤微生物PLFA组成、含量、微生物群落结构及多样性的差异性，从微生物群落水平解析无致病力青枯雷尔氏菌对烟草青枯病的免疫机制。结果表明，与对照处理相比，无致病力青枯雷尔氏菌处理增加烟草根系土壤i17:1ω9c和17:1ω8c两种PLFA，对烟草根系土壤PLFA总含量的影响呈现先降后升趋势。无致病力青枯雷尔氏菌处理能改变烟草根系土壤微生物群落结构，促进细菌和真菌的生长，抑制放线菌的生长，降低G+/G-的比值，促进烟草根系土壤G-菌的富集，增加烟草根系土壤微生物群落结构多样性。主成分析表明，前5个主成分可解释93.31%的PLFA变异。第一主成分可解释PLFA 50.31%的变异，第二主成分可解释16.60%的变异。无致病力青枯雷尔氏菌处理15d的烟草根系土壤PLFA在主成分一和主成分二上均有较高的载荷值，且与其它处理相距最远，说明无致病力青枯雷尔氏菌处理15d时烟草根系土壤微生物结构变异最大。聚类分析表明，基本上可将无致病力青枯雷尔氏菌处理不同取样期的烟草根系土壤和清水对照处理不同取样期的烟草根系土壤分别聚在两大不同的类群中，说明无致病力青枯雷尔氏菌能明显改变根系土壤微生物群落结构。 关键词：无致病力青枯雷尔氏菌；根系土壤微生物群落结构；磷脂脂肪酸 Effect of avirulent strain of Ralstonia Solanacearum on soil microbial community structure in root system of tobacco with the method of PLFA ZHENG Xuefang, LIU Bo*, LAN Jianglin, ZHU Yujing, CHE Jianmei, SU Mingxing Agricultural Bio-Resources Research Institute, Fujian Academy of Agricultural Sciences, Fujian Fuzhou 350003 Abstract: In order to study the effects of c of Ralstonia Solanacearum on soil microbial community structures, soil of tobacco root system among sampling times was determined by phospholipid fatty acid (PLFA). The results showed that avirulent strain of Ralstonia Solanacearum treatment increased two PLFA that is i17:1ω9c and 17:1ω8c while comparing to the control. Amount of PLFA for soil under virulent strain treatment was decreased at first then increased subsequently.Soil microbial community structure had been changed due to avirulent strain of Ralstonia Solanacearum treatment, and the rhizospere biomass of bacterial and fungal and G+/G- were increased, while the population of actinomycetes was decreased. Moreover, avirulent strain of Ralstonia Solanacearum treatment increased the diversity of microbial communities in the tobacco rhizosphere. Principal component analyses of 20 PLFA in whole soil were conducted to determine microbial community structu