流式细胞术检测CFSE标记人T细胞亚群增殖反应_王敏.pdfVIP

21 5 2005 9 IMMUNOLOGICAL JOURNAL Vol. 21 No . 5 Sept. 2005 423 [ ]2005) CFSET 1 1 1 2 2 1, 2 * 王 敏 , 张林杰 , 何金生 , 葛晓松 , 陈 勇 , 李柏青 ( 1. 安徽医科大学免疫学教研室, 合肥 230001; 2. 蚌埠医学 免疫学教研室, 安徽蚌埠233003) [ ] CFSE T (PBMC) CFSE , ( PHA) CD3 mAb ( MtbAg) , IL2 , T , ModFit PHA CD3 mAb + + + + T , CD8 T CD4 T , CD4 T 4 CD8 T MtbAg T CFSE , T [ ] ; ;T ; [ ] R392. 12 [ ] A Determination of the proliferation of CFSE labeled human T lymphocyte subpopulations by flow cytometry WANG Mi , ZHANG Li jie, HE Ji she g, GE Xiaoso g, CHEN Yo g, LI Baiqi g( Dep artment of Immunology , A nhui Medical University , Hef ei 230001, China) [Abstract] Objective To evaluate the method of determi i g the proliferatio of huma T lymphocyte subpopulatio s by usi g flow cy tometry a d carboxyfluorescei diacetate succi imidyl ester( CFSE) labeli g assay. ethods PBMC isolatedfrom ormal subjects were labeled with CFSE, a d stimulatedwith PHA, CD3 mAb, a d MtbAg, respectively . The , the stimulated PBMCwas cultivated i medium co tai i g IL2. The phe otypes of proliferated lymphocyteswere determi ed by flow cytometry. The dy amics of the proliferatio of activatedT cell sub populatio s were a alyzedwith the ModFit software. Results Total T cells were mostly activated by PHA a d CD3Ab. Differe t proliferatio s + + + were observed betwee CD4 T a d CD8 T cells, i which CD8 T cells had greater reproductio . O the other ha d, MtbAg was mai ly stimulated the reproductio of the T cells. Conclusion With assista ce of CFSE labeli g assay , the flow cytometry is able to obtai more detail i formatio of proliferative respo se a d dy amics of differe t T cell subpopulatio s after stimulated with differe t stimuli. [Key words] Carboxyfluorescei diac