动物源性大肠埃希菌超广谱β-内酰胺酶的检测及耐药性研究.pdfVIP

窑论 著 窑 中国当代医药 2014年 12月第21卷第35期 动物源性大肠埃希菌超广谱 β-内酰胺酶的检测 及耐药性研究 周敬纲 吉林省公主岭市疾病预防控制中心袁吉林公主岭 136100 [摘要] 目的了解动物源性大肠埃希菌超广谱茁-内酰胺酶渊ESBLs冤的检出率和耐药特点袁为其耐药性的安全评价 提供依据遥 方法 采用NCCLS推荐的表型检测法和确认试验测定大肠埃希菌ESBLs袁纸片扩散法测定大肠埃希 菌的耐药性遥 结果 222株大肠埃希菌对环丙沙星渊83.78%冤尧诺氟沙星渊92.79%冤尧利福平渊81.08%冤的耐药率很 高曰对阿米卡星尧亚胺培南高度敏感遥ESBLs检出率为 12.16%渊27/222冤袁产ESBLs菌株除对亚胺培南外其余 13种 抗生素的耐药率均高于非产ESBLs菌株遥 结论动物源性大肠埃希菌对很多抗生素具有一定的耐药性袁 应加强 对食品中动物源性大肠埃希菌耐药性的监测遥 [关键词] 大肠埃希菌曰动物源食品曰耐药性 + [中图分类号] R378.21 [文献标识码]A [文章编号] 1674-4721渊2014冤12渊b冤-0008-04 ESBLs detection and drug resistance study on Escherichia coli of animal origin ZHOUJing-gang Centerfor Disease ControlandPreventionof Gongzhuling CityinJilin Province,Gongzhuling 136100,China [Abstract] Objective To investigate the detection rate and the drug resistant characteristic of ESBLs on Escherichia coli of animal origin in order to provide a basis for the safety evaluation of drug resistance. Methods ESBLs of Es鄄 cherichiacoli was detected by phenotype detection method and confirmatory test recommended by NCCLS.Drug resis鄄 tance of Escherichia coli was detected by disk diffusion method. Results The drug resistant rates of 222 Escherichia coli strainsonciprofloxacin(83.78%),noroxin(92.79%)andrifampicin(81.08%)were quite high,while the strainswere high sensitive to amikacin and imine culture south.The detection rate of ESBLs was 12.16%(27/222).Inaddition to imipen鄄 em,the drugresistant rate of ESBLs-producing strains on 13kindsof antimicrobials was much higher than that of non- ESBLs-producing strains respectively. Conclusion Escherichia coli of animal origin have certain resistance to a great many antibiotics.It should strengthenthe monitoringof drugresistance onEscherich