替沙星体外抗生素后效应期间大肠埃希菌形态学变化研究.pdfVIP

加替沙星体外抗生素后效应期间大肠埃希菌形态学变化研究 王睿 朱曼 张永青 梁蓓蓓 中国人民解放军总医院临床药理药学研究室 北京 100853 [摘要] 目的 通过对加替沙星体外 PAE 期间 E.coliATCC25922 的形态学观 察以及菌体大小 DNA 含量变化的研究 探讨 PAE 形成机制 方法 于加替沙 星体外 PAE 期间不同时间点吸取培养物 应用原子力光显微镜观察 E.coliATCC25922 的形态学变化 采用流式细胞仪技术与荧光探针碘化丙啶结合 检测其菌体大小 DNA 含量变化 结果 原子力显微镜结果表明E.coliATCC25922 在菌落计数法所测定的 PAE 之后仍有丝状体存在 流式细胞仪分析结果表明 与空白对照组比较 其体外PAE 期间E.coli ATCC25922 菌体显著延长 DNA 含 量变化显著升高 结论 加替沙星体外PAE 期间E.coli ATCC25922 呈特征性的丝 状体变化 而其菌体大小 DNA 含量变化随之相应变化 提示丝状体形成与DNA 含量变化相关 [关键词] 加替沙星 抗生素后效应 大肠埃希菌 The change of sizes and nucleic acid contents of Escherichia coli during the Postantibiotic effect after exposure to gatifloxacin and ciprofloxacin Wang rui, Zhu man, Zhangyongqing, Liangbeibei (Department of clinical pharmacology, Chinese PLA General Hospital Beijing 100853 ) ABSTACT: OBJECTIVE We study the change of sizes and nucleic acid contents of Escherichia coli during the Postantibiotic effect after exposure to gatifloxacin in order to investigate the mechanism of PAE. METHODS During the Postantibiotic effect after exposure to gatifloxacin, the aliquots were taken from the bacterial culture at regular interverals. Then We used atomic force microscope to observe changes in bacterial ultrastructure , then employed flow cytometry in conjunction with fluorescent probes to follow the dynamic change of sizes and nucleic acid contents of Escherichia coli. Results The sizes of Escherichia coli were different from those of the control population. In parallel, an increase in nucleic acid contents was still noted at the end of tne experiment. This change was outlasting the classically defined PAE. CONCLUSION Gatifloxacin induced filamentation and the increase of nucleic acid contents of Escherichia coli, these observation suggest that the filamentation may be a result of