层层自组装法制备碳纳米管_酶多层膜电极及其检测酚类物质.pdfVIP

2009 年第 67 卷 化 学 学 报 Vol. 67, 2009 第 24 期, 2857～2861 ACTA CHIMICA SINICA No. 24, 2857～2861 •研究论文• 层层自组装法制备碳纳米管/酶多层膜电极及其检测酚类物质 杨绍明* 黄爱花 魏志鹏 江 丹 郑龙珍 (华东交通大学基础学院化学化工系 南昌 330013) 摘要 将十二烷基磺酸钠(SDS)分散的碳纳米管(CNT)和辣根过氧化物酶(HRP)通过层层自组装方法构筑HRP 多层膜酶 电极, 并将其用于酚类物质的分析检测研究. 紫外-可见光谱表明: SDS 分散的 CNT 可与 HRP 均匀有效地组装构筑多 层酶膜. 电化学研究表明: CNT 的引入很好地提高了HRP 电极的灵敏度; 随着CNT/HRP 组装层数的增加, 电极的电化 学响应增加. 研究结果表明该 HRP 电极对酚类物质(邻苯二酚、对苯二酚和苯酚) 的分析检测具有宽的线性范围、好的 灵敏度和抗干扰性. 关键词 碳纳米管; 辣根过氧化酶; 酚; 层层自组装 CNT/Enzyme Multilayer Electrode Prepared by Layer-by-lyer Self-assembly and Its Detection for Phenols Yang, Shaoming* Huang, Aihua Wei, Zhipeng Jiang, Dan Zheng, Longzhen (Department of Chemistry and Chemical Engineering, East China Jiaotong University, Nanchang 330013) Abstract A horseradish peroxidase (HRP) multilayer enzyme electrode was constructed with carbon nanotubes (CNT) dispersed by sodium dodecylsulfonate (SDS) and HRP via layer-by-layer (LBL) self-assembly. UV-Vis spectroscopy and SEM were used to monitor the uniform LBL self-assembly of CNT and HRP. With catechol as substrate, electrochemical study showed that the use of CNT could obviously improve the sensitivity of the HRP electrode, and the response of the electrode increased with the increment of the CNT/HRP bilayer. The performances of the (CNT/HRP)4 electrode were studied for the phenols de- termination. The experimental results showed a wide linear range, good sensitivity and anti-interference ability when the HRP electrode was applied to the determination of phenols (