16S-23S+rDNA内转录间隔区序列寡核苷酸探针鉴定分支杆菌菌种的研究.pdfVIP

中闻跨寿嘉志2002年10月第24卷增刊 Naturalresistancetointfectionwithintraeellular relatedto the yeastrrsanganesetransporter 1 isrecruitedtothe involvedinconferring pathogens：theNrampprotein nmcrophageprotein Natl Sci membraneofthe Med to Acad USA， phagosome[J]．Exp1997， myocbaeteria[J]Proc 185：717—730 1996，93：5105—5110 L，Nelsona1．A [10]SupekF，Supekova H，et 16S．23S rDNA内转录间隔区序列寡核苷酸探针 鉴定分支杆菌菌种的研究 解放军第三。九医院100091李国利庄玉辉赵铭 中国药品生物制品检定所100050王国冶陈保文沈小兵 摘要：目的研究以16S-23SrDNA内转录问隔区 internaltranscribed 向杂交技术检测26种36株分支杆菌标准株，24株分支杆菌临床分离株。结果分支杆菌标准株和临床 寡核苷酸探针除草分支杆菌探针MPH与微黄分支杆菌亦杂交外，其余探针均为特异的。5株结核分支杆 菌I临床分离株箍定为结核分支杆菌复合群，19株非结核分支杆菌临床分离株鉴定至种水平。结论168- 23SrDNAITSPCR坂向杂交鉴定分支杆菌菌种简便、快速、准确，具有较高应用价值。 关键词：分支杆菌；鉴定；16S-23SrDNA内转录间隔区；寡核苷酸探针；聚合酶链反应一反向杂 交 on identificationof Study speciesusing mycobacterum basedOil16s-23srDNAinternal oligonucleotideprobes transcribed spacersequences LI a1． Gno-li。ZHUANGYu-hui，ZHAOMing，et The309 of HospitalPLA，Beijing100091， To the identificationOf Abstract：Objectivestudy rapid mycohaeteriumspeciesby basedon16s_23SrDNAinternaltranscribed hybridizationassayusingoligonucleotideprobes 36 of standardstrainsfrom26 and24clinicalisolates sequences．Methods myeobacterialspecies DNA were from weredetectedPCR—reverse fragmentsamplified by hybridizationassay．Results340bp--450bp all strains 21 tested．Amongoligonucleotide mycobaeterial andMSMwere for SPP．M．mbereulosis specificrespectivelymyeobacterium wit 1 and MPHshowedahybridization abscessus，M．vaccaeM．smegmatis．Theprobe isolatesof tubereukxsisweredeterminedtobeM．tuberculosis clinical clinical mycobacterium complex．19 at 1evel．Conclusion imlatesofnontuberculouswereidentilled mycobactaria mycobacteriurnspecies 中闻防寤杂志2002年10月第24卷增刊 in identificationof rDNAITSPCR—reverse isofvalue hybridization simple，rapid，accuracy species． rDNAintern,dtranscribed Keywords：Mycobacterium；Identification；16s一23s reverse probes；PCRhybridizaion 近年来，在世界范围内非结核分支杆菌 摩、鸟、胞内、蟾蜍、胃、土地、次要、偶然、 NTM 病发病率明显增加，我国全国结核病流行龟、脓肿、母牛、耻垢和草分支杆菌探针MKA、 病学抽样调奁 流调 结