永生化的骨髓基质细胞移植对损伤脊髓的保护作用.pdfVIP

2006 7 8 Pra ctical Clinica l Med icine , 2006, Vol 7, o8 # 7 # , , ( 广东医学院第一临床学院外科学教研室, 广东湛江524001) : : ( Bone marrow st em cells, BMSCs) : SD , ( A ) BMSCs ( B ) ( Ner e grow th fac- tor, NGF) ( Brain Deri ed ner e growt h fact or, BDNF) BMSCs ( C ) ( D ) 24 h 8 , 612 , 8 , ( MEP) : ( SCI) , + 2+ + 2+ Na Ca , K Mg NGFBDNF , SCI : NGFBDNF BM SCs SCI , : ; ; ; : R- 332 : A : 1009- 8194( 2006) 08- 0007- 03 Protective Effects of Intracord Transplantation of Immortalized Bone Marrow Stem Cells on Spinal Cord Injury ZHO G Ha-i hong, BU Lin, CHE J-i ming (D eap rtment of Surgery , First Clinical H osp ital of Guangdong Medical College Zhanj iang 524001, China) Abstract:Objecti e: To in estigate the protecti e effects of intr acord transplantation of immorta-l ized Bone marrow stem cells on spinal cord injury . M ethods: Spinal cord injured r ats w ere di ided into 3 groups. Gr oup C consisted of the r at w ith treated w ith the transplantation of modified NGFBDNF genes BMSCs( after immortalized) ; group B of the rats w ith transplantation of BM- SCs w ithout . immortalized; A t 24 h after operation, 8 r ats of each gr oup w er e killed and the in- juered segment of the spinal cord w as resected to be examined w ith atomic absorption spectropho- tometry . At 6 w eeks and 12 w eeks after transplantation, the another r ats w ere ex amined w ith neu- r ological function test and M EP monitor ing. Results: there w as a significant incr ease of w ater con- + 2+ + 2+ tant and Na Ca ions and decreased of K M g ions in the injured cor d segment of gr