2015 January.DOCVIP

* 2005 January From richard at cing.ac.cy Tue Jan 4 07:30:43 2005 From: richard at cing.ac.cy (Richard Mean) Date: Tue Jan 4 07:36:57 2005 Subject: [tg-l] Puromycin plasmid request. Message-ID: 41DA4623.99281AAC@cing.ac.cy Dear List, First of all, Happy New Year for 2005, hope everybody had a good Christmas. I have a favour to ask, does anybody have a plasmid (any plasmid) containing the puromycin gene (ideally with an IRES in front) that they could let me have some of, as I need it to generate a stable cell line. Be very grateful if someone could help me out. Many thanks Richard Richard Mean Transgenic Facility Developmental and Functional Genetics Laboratory The Cyprus Institute of Neurology and Genetics 6 International Airport Avenue Ayios Dhometios Nicosia 1683 Cyprus Tel: +357Fax: +357e-mail: richard@cing.ac.cy ************************************************************************************************** The contents of this email and any attachments are confidential. It is intended for the named recipient(s) only. If you have received this email in error please notify the system manager or the sender immediately and do not disclose the contents to any one or make copies. ** CING Security System scanned this email for viruses, vandals and malicious content ** ************************************************************************************************** From chris.jackson at bristol.ac.uk Tue Jan 4 11:47:24 2005 From: chris.jackson at bristol.ac.uk (Chris Jackson) Date: Tue Jan 4 11:51:44 2005 Subject: [tg-l] Conditional Knockouts Message-ID: GMEJINJAAEMOIGKBNFGNCEBADLAA.chris.jackson@bristol.ac.uk Dear List, Im sorry to ask what is probably a pretty dumb question, but is there any simple way of converting a normal knockout mouse into a conditional knockout? Regards, Chris Jackson. -- Bristol Heart Institute University of Bristol Level 7, Bristol Royal Infirmary Bristol BS2 8HW United Kingdom. Voice/Fax: +44 (0) 117 928 2534