直投式青海弧菌冻干粉保护剂的筛选及优化.pdfVIP

网络出版时间：2012-05-28 08:37 网络出版地址：/kcms/detail/11.1759.TS0837.010.html 直投式青海弧菌冻干粉保护剂的筛选及优 化 1 * 黄盈盈 ，丁武 （西北农林科技大学食品科学与工程学院，陕西杨凌712100 ） 摘要：为了得到直投式青海弧菌 Q67 在冷冻干燥过程中最好的保护剂，首先单因素筛选效果较好的保 护剂，再采用响应面分析法对青海弧菌 Q67 发光菌冻干保护剂进行优化研究。结果表明：脱脂乳、蔗糖、 可溶性淀粉、谷氨酸钠对菌种 Q67 保护作用突出，以冻干后菌粉复苏30min 的相对发光率(RLU)为响应值， 当脱脂乳 7.68%，蔗糖 2%，可溶性淀粉 0.25%，谷氨酸钠 1%作为混合保护剂使用时，能使青海弧菌的相 对发光率高达 1382.87。并当保护剂：菌体=1:1 和作用时间为 30min 时，青海弧菌的相对发光度高达 1356。 关键词：直投式；青海弧菌 Q67 ；冷冻干燥；保护剂；响应面分析 Screening and optimization of the freeze-dried protectants for the lyophilized Vibrio-qinghaiensis of direct vat set * Huang Ying-ying，Ding u (College of Food Science and Engineering , Northwest AF University, Yangling, Shanxi 712100) Abstract: In order to obtain the best protectants for Vibrioqinghaiensis sp.-Q67 of direct vat set, the freeze-dried protectants was firstly screened by the single factors and then optimized by response surface analysis. The studies indicated that in many substances the skim milk, saccharose, soluble starch and sodium glutamate have outstanding protective effct. In response to The relative light unit(RLU) of the lyophilized Vibrioqinghaiensis sp.-Q67 after the regeneration for 30min, when taken skim milk 7.68%, sucrose 2%, solub