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Influenceofthephysico-chemicalfactors,plantgrowth:的物理化学因素的影响,植物生长.pdf
Influence of the physico-chemical factors, plant growth regulators, elicitors
and type of explants on callus cultures of medicinal climbers of Passiflora L.
MARCIN OŻAROWSKI1,2
1 Department of Pharmaceutical Botany and Plant Biotechnology
University of Medical Sciences
Św. Marii Magdaleny 14
61-861 Poznań, Poland
2 Department of Pharmacology and Experimental Biology
Institute of Natural Fibres and Medicinal Plants
Libelta 27
61-707 Poznań, Poland
corresponding author: e-mail: mozarow@.pl
S u m m a r y
This paper reviews the available research results published in 2006–2011. The attention
has been focused on biological processes in the in vitro callus cultures which were obtained
from various species of medicinal climbers of Passiflora . Procedures of callus induction
on different parts of plants including physico-chemical conditions, among others light,
photoperiod, temperature, basal medium, concentration and composition of plant growth
regulators: auxin – 2,4-dichlorophenoxyacetic acid (2,4-D), 1-naphtaleneacetic acid (NAA),
picloram – PIC (4-amino-3,5,6-trichloro-2-pyridinecarboxylic acid), cytokinin – benzylade-
nine (BA), kinetin (KIN), thidiazuron (TDZ), and gibberellin GA3 were analyzed. The occur-
rence of complex interactions between these factors and the efficiency of callus induction
as well as its proliferation and development were described. The experiments in callus
culture (induction of somatic embryogenesis, indirect organogenesis, cell suspension cul-
tures) including studies concerning the effect of the elicitor (methyl jasmonate, MeJA) and
the precursor (L-tryptophan) as well as phytochemical investigations were summarized.
It has been found on the basis of reviewed bibliography for a systematic research in this
area.The important role of callus cultures in search of an alternative source of p
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