活化后血小板对小鼠巨噬细胞分泌肿瘤坏死因子和凋亡的影响.pdfVIP

2010 6 15 3 J China Pediatr Blood Cancer, June 2010, Vol 15, No. 3 12 陈建军 徐之良 ! 初步探讨活化后血小板 ( PLT)对小鼠巨噬细胞分泌肿瘤坏死因子 (TNF)及 凋亡的影响 体外培养小鼠巨噬细胞系RAW 264 细胞, 用 10 mg/L LPS + 20 U /m l 4 IFN激活 RAW 264 细胞, 分别用 1∀ 10 /ml 1∀ 10 /m l活化后 PLT刺激被激活的RAW 264 细 胞, TNFE lisa试剂盒检测细胞培养上清中TNF水平, 流式细胞术分析细胞凋亡情况 经活化后 PLT刺激 24 h激活的RAW 264 细胞培养上清中TNF含量明显增加; 活化后 PLT 刺 激 6 d, 凋亡明显被抑制 活化后 PLT可促进小鼠巨噬细胞分泌TNF并抑制 凋亡 ! 活化血小板; 巨噬细胞; 凋亡; 肿瘤坏死因子 E ffect of activated p latelets on apop tosis and the tum or n ecrosis factor releasing of m ou se m acrophages Chen J ianjun, X u Zh iliang. Department of Ped iatrics, Renm in Hosp ital of Wuhan Un iversity, Wuhan 430060, China Abstract! Objective To exp lore the influence of activated platelets on apoptosis and the tumor necrosis factor ( TNF) releasing of mouse macrophages. M ethods Mouse macrophage cell line RAW 264 cellsw ere cu ltured in vitro, activated w ith 10 mg /L lipopolysaccharide ( LPS) + 20 U /m l 4 interferon ( IFN ). Then stmi ulate RAW 264 cells w ith 1 ∀ 10 /m l and 1 ∀ 10 /m l activated platelets, detect TNF levels in cell culture supematant w ith TNF detection ki,t flow cytometry analysis cell apoptosis. Results TNF contents in supematant significantly increased after 24 hours treatment of activated platelets, and apoptosisw as inh ib ited significantly after 6 days treatment of activated p latelets. Conc lu sion Activated platelets can promote secretion of tumor necrosis factor and inh ib it apoptosis of mousem acrophage. Key w ord s! A ctivated p latelets; M acrophage; Apoptosis; Tumor necrosis factor ( PLT), , ,