7个微卫星座位与北京荷斯坦母牛体细胞评分关系的研究.pdfVIP

A cta Genetica S inica, M ay 2005, 32 ( 5): 471~ 475 ISSN 0379- 4 172 Study on Relationships Between Seven M icrosatellite Loci and Somatic CellScore in Beijing Holstein Cows 1, 2 3 4 4 CHU M ingX ing , ZHOU GuoLi, JIN HaiGuo , SH IW anHai, Cao FuCun , 1 1 2 FANG L i, YE SuCheng , ZHU Yan ( 1. Institute of A nmi al S cience, Ch ineseA cademy of Agricultura l Sc iences, Bejiing 100094, China; 2. Colleg e of Lfi e S cience, L iaoch eng University, L iaocheng, Shandong 252059, Ch ina; 3. De artm ent of A nima l Science, College of Agriculture, Yan bian University, Longj ing, Jilin 133400, China; 4. B eij ing Da iry Cattle Centre, B eij ing 100085, China) Abstract: Genetic variation of seven microsatellite lociBM 1818, BM 1258, BM 1443, BM 1905, BM 302, BM 4505 and C YP 21 w hichwere closely linked to somatic ce ll score ( SCS) was analyzed in 240 Beijing Holstein cows w ith nondenaturni g polyacryal m di e gel e el ctrophoresis. lel le frequencies, heterozygosity, polymorphic informatoi n content, the effective numberofalleel s ofseven microsatellite ol c iwere calcual ted. Relationships between seven m icrosatellite lociand somatic cellscore in Beijing Holstein cowsw ere prmi arily anayl zed by el astsquares linear mode.l Leastsquaresmeans ofSCS forBM 1818 (284 bp/284 bp), BM 1258 ( 106 bp/92 bp), BM 1443 ( 166 bp/ 160 bp), BM 1905 (187 bp/187 bp), BM 302 ( 142 bp/140 bp),BM 4505 (240 bp/236 bp) andCYP 21 (215 bp/198 bp) were real tively lower, and theirgenotypesw ere the most favorable genotypes ni respect