Akirin2基因在肉鸭胸肌和腿肌中的发育表达研究.docVIP

Akirin2基因在肉鸭胸肌和腿肌中的发育表达研究 王万霞，黄锎靓，刘贺贺，代 飞，寇 洁，李 亮，王继文 （四川农业大学动物类实验示范中心，四川雅安 625014） 摘 要：Akirin基因家族中Akirin2基因与哺乳动物肌纤维类型密切相关。鸟类中仅有Akirin2基因，为研究Akirin2与鸭肌纤维类型的关系，本文比较了鸭10、14、18、22、27日胚龄及出生后1周龄（P7 d）胸肌和腿肌的发育差异，并采用QRT-PCR法检测了Akirin2在以上各阶段胸肌、腿肌中的发育性表达。结果表明，Akirin2基因的表达与胸肌肌纤维的发育关系密切，其表达的增高伴随肌纤维直径的增粗；相反，在腿肌中Akirin2的表达降低伴随肌纤维直径的增粗。因此，Akirin2基因与鸟类红肌纤维发育关系密切，其有可能是鸭胸肌发育以及肌纤维类型组成的关键基因之一，值得在今后的基础研究和育种研究中引起重视。 关键词：Akirin2；胸肌；腿肌；QRT-PCR；肌纤维发育 Expression of Akirin2 Gene in Development of Breast and Leg Muscle in Duck WANG Wanxia，HUANG Kailiang，LIU Hehe，DAI Fei，KOU Jie，LI Liang，WANG Jiwen （Animal Experimental Demonstration Center，Sichuan Agricultural University，Ya′an，Sichuan 625014） Ａｂｓｔｒａｃｔ：The Akirin2 gene belongs to the Akirin gene family in mammals，while there is only Akirin2 gene in birds. The Akirin2 has a close relationship with the muscle fiber type differentiation in mammals，in order to know the relationships between Akirin2 gene and muscle fiber types in birds，the developmental differences between breast muscle and leg muscle of ducks during embryonic stage 10，14，18，22，27 days and 7 days postnatal age were compared，and mRNA expression of Akirin2 in these tissues of developmental stages were detected using QRT-PCR method. The results showed that developmental expression level of Akirin2 had a close relationship with duck muscle fiber development. The increasing expression level of Akirin2 accompanied with the increasing of breast muscle fiber diameter， while it was just the opposite in leg muscle. Therefore，Akirin2 gene may play more important roles in breast muscle than leg muscle，and it might be one of the key factors influencing breast muscle growth and differentiation. That deserved a more great attention in fundamental study and animal breeding research in the future. Key words：Akirin2；breast muscle；leg muscle；QRT-PCR；muscle fiber development 肌肉形成是一个复杂的过程，其主要由来自轴旁中胚层和侧中胚层的成肌细胞，逐步发育成多核肌管，最终融合形成成熟的肌纤维［1］。在肌肉发育过程中，许多基因直接或间接地参与了调控。Akirin基因在进化过程中形成2个同源基因，分别命名为Akiri