IP6诱导HT_29细胞凋亡中线粒体通路的实验研究_张晓妮.pdfVIP

2009 8 24 4 M ed J Q ilu, A ugust 2009, V ol . 24, N o . 4 # 3 19 # doi:10. 3969/ j . issn . 1008- 0341. 2009. 04. 014 # # IP6 H T- 29 1 2 1 , , ( 1 , 26602 1 2 ) [ ] bcl- 2 C( Cy t- C) Ca2+ ( IP6) HT-29 3 . 3 mmol/ L IP 6 3 d 6 H T- 29 , IP6 H T- 29 RT- P CR IP6 bc-l 2 m RN A , Cyt- C , Fura- 2 I P6 HT-29 Ca2 + , IP 6 bcl- 2 , Cy t- C Ca2 + IP6 H T- 29 , bc-l 2Cyt- CCa2 + [ ] HT- 29 , bcl- 2 C [ ] R735. 35 [ ] A [ ] 1008-0 34 1( 2009) 04- 03 19- 03 EXPERIMENTAL STUDY OF MITOCHONDRIAL PATHWAY IN IP6- INDUCED HT- 29 CELL APOPTOSIS ZH A N G X IA O-NI , L EN G CH UA N-LI , S ON G YA N G ( Ins tit ut e of N ut rit ion and F ood H y gien e, Qin gdao U niv er sit y M edical Coll ege , Q ingdao 26602 1, China) [ABSTRACT] Obj ect ive T o inv est igat e t he rol e of mit och ond rial pat hw ay in wh ich bcl- 2, Cyt ochrome- c ( Cyt- C) and Ca2+ part ici pat ed in ino sit ol hex aph o sphat e ( IP 6) induced apopt o sis of HT- 29 human colon carcinoma cell line . Met hod s H T- 29 cells w ere ex po sed t o t he sam e concent rat ions of IP 6 ( 3. 3 mmol/ L) for dif ferent t ime: t hree day s or s ix m ont hs. RT-P CR w as u sed t o d et ect t h e ex pressi on of b c-l 2, an d immun ocyt ochem ical st ain used t o t est t h e lev el of Cyt- C, Fura- 2/ A M t o assay t he concent rat ion of int racell ular-f ree calcium . Result s Compared w it h t