纳米金制备方法.pdfVIP

Int. J. Mol. Sci. 2007, 8, 526-532 International Journal of Molecular Sciences ISSN 1422-0067 © 2007 by MDPI /ijms Full Research Paper A Centrifugation-based Method for Preparation of Gold Nanoparticles and its Application in Biodetection Zhiqiang Liang 1,2, Juan Zhang 2, Lihua Wang *,2, Shiping Song 2, Chunhai Fan 2 and Genxi Li *,1 1 Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China; E-mail: suyuanyuan@ (Zhiqiang Liang); genxili@ (Genxi Li) 2 Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China; E-mail: suyuanyuan@ (Juan Zhang); wanglihua@ (Lihua Wang); songshiping@ (Shiping Song); fchh@ (Chunhai Fan); * Authors to whom correspondence should be addressed; E-mails: genxili@, wanglihua@ Received: 18 April 2007; in Revised Form: 11 May 2007 / Accepted: 15 May 2007 / Published: 13 June 2007 Abstract: Gold nanoparticles (AuNPs) have found widespread applications in life sciences. While synthesis of monodispersed AuNPs has been fairly convenient by using chemical reduction of chloroauric acid by sodium citrate, we found that AuNPs of high quality and high concentrations were not readily obtained via this method. As an example, we showed that monodispersed 13-nm AuNPs were readily synthesized at relatively low concentrations (e.g. 3.5 nM); in contrast, 13